Microbial Succession in the Cheese Ripening Process-Competition of the Starter Cultures and the Microbiota of the Cheese Plant Environment.

A large variety of cheeses can be produced using different manufacturing processes and various starter or adjunct cultures. In this study, we have described the succession of the microbial population during the commercial production and subsequent ripening of smear-ripened cheese using 16S rRNA gene sequencing. The composition of the microbiota during the first 6 days of production was constant and consisted mainly of LAB (lactic acid bacteria) originating from the starter culture.

First Evidence of the Presence of the Causative Agent of Caseous Lymphadenitis- in Dairy Products Produced from the Milk of Small Ruminants.

This study focused on the detection and quantification of selected bacteria and on the presence of enterotoxin genes in milk and dairy products from sheep and goat farms in the Czech Republic using quantitative real-time PCR (qPCR) and multiplex PCR (PCR). The presence of (CP), subsp. (MAP), , , enterotoxin genes and methicillin-resistant (MRSA) was determined in 18 milk samples, 28 fresh cheeses, 20 ripened cheeses and 14 yoghurts.

Effect of diluent on the extraction of europium(iii) and americium(iii) with ,,','-tetraoctyl diglycolamide (TODGA).

Solvent extraction of Eu and Am ,,','-tetraoctyl diglycolamide (TODGA) dissolved in different molecular diluents was studied. The diluent types used in this work were primary and secondary alcohols, secondary ketones and alkanes. Effects of concentration of extracting agent, temperature, diluent type and its carbon chain length on the extractions were determined.

Detection and molecular characterisation of methicillin-resistant Staphylococcus aureus isolated from raw meat in the retail market.

Objectives: This study aimed to detect and characterise methicillin-resistant Staphylococcus aureus (MRSA) from retail meat in the Czech Republic.

Methods: Isolates were identified by PCR detection of the S. aureus-specific fragment Sa442 and mecA gene.

A cross-border outbreak of Bareilly cases confirmed by whole genome sequencing, Czech Republic and Slovakia, 2017 to 2018.

In August 2017, an increased incidence of Bareilly was detected in the Czech Republic. An investigation was conducted with Slovakia to confirm the outbreak and identify the source. Probable outbreak cases were defined as cases with laboratory-confirmed Bareilly reported in either of the national surveillance systems, and/or the Czech and Slovak National Reference Laboratory databases from July 2017.