[Creation of a hybrid protein gene based on Bacillus thuringiensis delta endotoxins CryIIIA and CrIA(a) and expression of its derivatives in Escherichia coli].

The 5'-terminal fragment (containing 1-565th codons) of Bacillus thuringiensis var. tenebrionis gene for the Coleoptera-specific delta-endotoxin CryIIIA was cloned. This sequence was extended with either only a homologous fragment of CryIA(a) or that one together with in-frame NPTII or GUS coding sequences.

[Dependence of the activity of phi X 174 B-promoter in the expression of Escherichia coli gal-operon on the number of its copies and their orientation].

Promoter activities of different restriction fragments of the R8 DNA region of phage phi X 174 were compared. The studied DNA fragments included HindII fragment R8 (B-promoter), its left portion 49 nucleotide long, and the central segment containing 113 nucleotides generated by AluI. The promoter activity of these fragments was quantitated by the appearance of uridyltransferase and galactokinase activities in Escherichia coli clones carrying plasmids pHD68-17.

[Discontinuous transfer of phage T7 DNA molecules into Escherichia coli cells during infection].

HpaI restriction analysis of the part of T7 DNA molecule which comes off from E. coli after ultrasonic desorption of virion had been carried out. In such a way it was possible to follow the transfer of labelled T7 DNA into the host cell after the phage adsorption under different conditions.

[Kinetic determination of the non-specific constants for the binding of Escherichia coli RNA polymerase to T7 phage DNA].

The kinetics of promoter complex formation of E. coli RNA polymerase with T7 phage DNA was studied under different ionic strengths at DNA concentrations 0.15, 1.