Modeling brain pathology to study nose-to-brain drug delivery.

Objectives: To create a new mucoadhesive dosage form based on PluronicF127 followed by transformation into a gel form upon intranasal administration for targeted delivery to brain tissueMETHODS: Citicoline, cytidine diphosphocholine, designated as CDP-choline, was purchased as a white powder with the molecular weight of 510.31 g/mol. The triblock copolymers of polyethylene glycol-block-polypropylene glycol-block-polyethylene glycol (PEG-PPG-PEG), branded as Pluronic F127, was used.

[The protective activity of the attenuated strain 15 of the Venezuelan equine encephalomyelitis virus in rodent infection via the respiratory tract].

Administration to rodents (Syrian hamsters, mice, guinea pigs, rabbits) of minimal doses of attenuated strain 15 VEE (up to 20 ImD50) provided protection against respiratory challenge with a highly virulent strain of Venezuelan equine encephalomyelitis virus. The protection is observed in a wide range of doses (up to LD50 hundreds and even thousands).

[The postvaccinal reaction in hamadryas baboons to the administration of attenuated strain 15 of the Venezuelan equine encephalomyelitis virus].

Immunization of Papio hamadryas with attenuated VEE virus (strain 15) causes seroconversion in 100% of animals. Postvaccinal reaction such as viremia and short-lasting fever occurs in response to the injection of 10(3) ImD50 in about 30% of animals. CNS lesion symptoms characterizing infection of Papio hamadryas with natural Trinidad strain do not occur in response to injection with attenuated strain.