[Adrenal adenoma secreting exclusively estrogens in a woman during her reproductive period. 11-year follow-up].

Feminizing tumours are rare, most are malignant, and they are usually seen in children and male adults. We report an 11-year follow-up of a feminizing adrenal tumour which was unusual on three accounts: it was a benign adenoma, it secreted estrogens only and the patient was an female in her active reproductive years. To our knowledge, similar cases have not yet been published.

[Evidence, in vitro, of estrone and estradiol-17 beta 3-sulfoconjugate hydrolysis in the uterus of pregnant guinea pigs].

The metabolism, in vitro, of [6,7-3H]-oestrone-3-sulfate and [6,7-3H]-oestradiol-17beta-3-sulfate by the uteri of pregnant female Guinea-Pigs has been studied using 900 g supernatants. A hydrolysis of the sulfate moiety was observed: 35% of the radioactivity was recovered in the unconjugated fractions after 1 hr. of incubation.

[3 alpha-hydroxy-5 alpha-androstane-17-one-3-sulphate in hirsute women's plasma (author's transl)].

The determination of serum androsterone-3-sulphate was carried out by radioimmunoassay with an antiserum specific against 15 alpha-carboxymethyl-androsterone-BSA. The values obtained (mean +/- SD) in ng/ml were 739 +/- 190 (n = 9) for Men; 285 +/- 157 (n = 13) for Women; 677 +/- 464 (n = 131) for hirsute women respectively and were compared to the values of urinary sulphate of dehydroepiandrosterone in the same subjects.

The preparation of three fluorescence-labeled derivatives of testosterone.

Three fluorescence-labelled derivatives of testosterone were prepared consisting of the steroid separated from the fluorochrome by a hydrocarbon "bridge". "Bridges" of different lengths (C2 to C7) were used as the length required to avoid steric hindrance effects by the fluorochrome in studies on steroid-protein binding was unknown. The three derivatives prepared were: 17 beta-hydroxy--4-androsten-3-one 3-(O-(N-(2'-mercapto)ethyl)carbamoylmethyl)oxime, 17 beta-hydroxy-4-androsten-3-one 3-(O-(N-(3'-amino)propyl)carbamoylmethyl)oxime and 17 beta-hydroxy-4-androsten-3-one 3-(O(N-(7'-amino)heptyl)carbamoylmethyl)oxime.