MKLP2 Is a Motile Kinesin that Transports the Chromosomal Passenger Complex during Anaphase.

During cytokinesis, signals from the anaphase spindle direct the formation and position of a contractile ring at the cell cortex [1]. The chromosomal passenger complex (CPC) participates in cytokinesis initiation by signaling from the spindle midzone and equatorial cortex [2], but the mechanisms underlying the anaphase-specific CPC localization are currently unresolved. Accumulation of the CPC at these sites requires the presence of microtubules and the mitotic kinesin-like protein 2, MKLP2 (KIF20A), a member of the kinesin-6 family [2-7], and this has led to the hypothesis that the CPC is transported along microtubules by MKLP2 [3-5, 7].

Untangling the contribution of Haspin and Bub1 to Aurora B function during mitosis.

Aurora B kinase is essential for faithful chromosome segregation during mitosis. During (pro)metaphase, Aurora B is concentrated at the inner centromere by the kinases Haspin and Bub1. However, how Haspin and Bub1 collaborate to control Aurora B activity at centromeres remains unclear.

Electrical Stimulation Frequency and Skeletal Muscle Characteristics: Effects on Force and Fatigue.

This investigation aimed to determine the force and muscle surface electromyography (EMG) responses to different frequencies of electrical stimulation (ES) in two groups of muscles with different size and fiber composition (fast- and slow-twitch fiber proportions) during a fatigue-inducing protocol. Progression towards fatigue was evaluated in the abductor pollicis brevis (APB) and vastus lateralis (VL) when activated by ES at three frequencies (10, 35, and 50Hz). Ten healthy adults (mean age: 23.

Functional electrical stimulation-induced muscular fatigue: Effect of fiber composition and stimulation frequency on rate of fatigue development.

This investigation evaluated the progression towards fatigue in two muscles of differing fast- and slow-twitch fiber proportions (abductor pollicis brevis (APB) and vastus lateralis (VL)) when activated by functional electrical stimulation (FES) at three frequencies (10, 35, and 50 Hz). Fatigue was defined as a 50% drop from the initial FES-induced force of 25% maximal voluntary contraction (MVC). Ten healthy adults (mean age: 23.

Baculoviral delivery of CRISPR/Cas9 facilitates efficient genome editing in human cells.

The CRISPR/Cas9 system is a highly effective tool for genome editing. Key to robust genome editing is the efficient delivery of the CRISPR/Cas9 machinery. Viral delivery systems are efficient vehicles for the transduction of foreign genes but commonly used viral vectors suffer from a limited capacity in the genetic information they can carry.