[Detection of toxin-producing pathogenic bacterial strains by polymerase chain reaction].

Polymerase chain reaction (PCR) was used for detection of pathogenic Clostridium botulinum, Clostridium perfringens, Clostridium difficile, and Escherichia coli. With this aim in view, primers to botulinic toxins types A, B, C1, D, E, F, and G, perfringens enterotoxin, difficile toxin, and types 1 and 2 Shigella-like toxins were chosen and synthesized. Optimal amplification conditions were selected for each pair of primers, with DNA and the respective agent as the reaction mixture matrices.

[The use of the polymerase chain reaction for the identification of Clostridium botulinum type A strains].

Polymerase chain reaction (PCR) for the identification of C.botulinum of type A was developed. As primers, oligonucleotides corresponding to sequences 913 -- 932 and 1852 -- 1871 of the gene of type A botulinic neurotoxin were used.

[1 of the causes of a change in viability of Escherichia coli L1 in air].

The possibility of changing the fatty acid composition of lipids in E. coli strain BB 20-14 by the introduction of ready lipid vesicles obtained from other E. coli strains into the growth medium has been established.

[Lethal processes in Escherichia coli due to dehydration in air with varying humidity].

The death of aerosolized bacteria has been shown to occur as the result of irreversible conformation changes in their macromolecules. This process depends on the role of cell dehydration and the amount of hydration water remaining in the cells.