dCasMINI-mediated therapy rescues photoreceptors degeneration in a mouse model of retinitis pigmentosa.

Retinitis pigmentosa (RP) is characterized by degeneration of rod and cone photoreceptors that progresses to irreversible blindness. Now, there are no mutation-agnostic approaches to treat RP. Here, we utilized a single adeno-associated virus (AAV)-based CRISPR activation system to activate phosphodiesterase 6B (Pde6b) to mitigate the severe degeneration in mice.

Enhancing Veterinary Education in Cambodia: Evaluation of Web-Based Resources in Teaching Herd Health and Epidemiology.

It can be challenging for veterinary schools in low- and middle-income countries (LMICs) to teach the 11 Competencies identified by the World Organisation for Animal Health (WOAH) due to inadequate faculty and teaching resources. This paper discusses the evaluation of web-based educational resources to support teaching in the Veterinary Faculty at the Royal University of Agriculture in Cambodia. Content- and pedagogy-based materials addressing herd health and epidemiology/disease investigation, their most urgent needs, were developed via a collaboration between Iowa State University, Ohio State University, and Massey University (New Zealand).

Clinical Characteristics and Treatment Outcomes in Unilateral Coats disease - a Global Collaborative Study.

Purpose: To evaluate the clinical outcomes and prognostic factors in unilateral Coats disease in the era of anti-VEGF therapy.

Design: Global, multicenter, retrospective case series.

Subjects: 656 eyes of 656 subjects with Coats disease were included in this study.

Discerning Emittable from Extractable Chemicals Identified in Consumer Products by Suspect Screening GCxGC-TOFMS.

Characterization of chemicals in household products is important for understanding this potential source of chemical exposure. Increasingly, suspect screening and nontargeted analysis techniques are used to characterize as many chemical signatures as possible. Solids such as household products are most conveniently prepared using solvent extraction, revealing what chemicals are contained within the product matrix but providing no information about the potential of those chemicals to leave the matrix and cause actual exposure.