Targeted isolation of Methanobrevibacter strains from fecal samples expands the cultivated human archaeome.

Archaea are vital components of the human microbiome, yet their study within the gastrointestinal tract (GIT) is limited by the scarcity of cultured representatives. Our study presents a method for the targeted enrichment and isolation of methanogenic archaea from human fecal samples. The procedure combines methane breath testing, in silico metabolic modeling, media optimization, FACS, dilution series, and genomic sequencing through Nanopore technology.

Binding of the transcription activator-like effector augments transcriptional regulation by another transcription factor.

DNA transcription is regulated by a range of diverse mechanisms and primarily by transcription factors that recruit the RNA polymerase complex to the promoter region on the DNA. Protein binding to DNA at nearby or distant sites can synergistically affect this process in a variety of ways, but mainly through direct interactions between DNA-binding proteins. Here we show that a Transcription Activator-Like Effector (TALE), which lacks an activation domain, can enhance transcription in mammalian cells when it binds in the vicinity of and without direct interaction with several different dimeric or monomeric transcription factors.

Measurement of polarization with the Degree Angular Scale Interferometer.

Measurements of the cosmic microwave background (CMB) radiation can reveal with remarkable precision the conditions of the Universe when it was approximately 400,000 years old. The three most fundamental properties of the CMB are its frequency spectrum (which determines the temperature), and the fluctuations in both the temperature and polarization across a range of angular scales. The frequency spectrum has been well determined, and considerable progress has been made in measuring the power spectrum of the temperature fluctuations.

Millimetric sky opacity measurements from the South Pole.

The opacity of the sky above the South Pole was measured in the millimetric atmospheric windows. We found the opacity to range from 0.1 to 0.

Cutting surfaces of revolution for millimeter wave optics.

A procedure for cutting surfaces of revolution using a numerically controlled milling machine is described. The procedure differs from conventional techniques in that the curve to be cut is approximated by a circular arc. The errors introduced by the approximation scheme are less than the machining errors.