Publications by authors named "M De Geyter"
Many studies have now confirmed that sperm DNA fragmentation (SDF) is associated with a poorer outcome of some forms of assisted reproduction technology. For this reason, SDF is an important parameter to evaluate in male fertility assessment. TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay coupled to flow cytometry is one of the most promising methods for SDF quantification.
View Article and Find Full Text PDFPurpose: Pregnancy-associated complications, duration of gestation and parity are well-known predictors of neonatal birth weight. Assisted reproductive technology (ART) affects neonatal birth weight as well. Endometrial thickness as measured on the day of HCG triggering may therefore impact on the neonatal birth weight.
View Article and Find Full Text PDFGene expression profiles of similarly derived human embryonic stem cell lines correlate with their distinct propensity to exit stemness and their different differentiation behavior in culture.
Cell Reprogram
June 2014
Article Synopsis
- Four human embryonic stem cell (hESC) lines were generated and maintained identically, yet showed differing gene expression patterns.
- These variations led to differences in their stemness and ability to differentiate into neurons, indicating some lines had a predifferentiated state at the start.
- The study suggests that lines that produced neurons faster might not be ideal for clinical use, as they might be less capable of proper and mature differentiation.
Objective: To develop a method to select spermatozoa with low DNA fragmentation rates for assisted reproduction technologies (ART).
Design: Multistep prospective cohort study.
Setting: University hospital.
Question Under Study: The feasibility and the potential advantages of separating X-chromosome bearing spermatozoa for the prevention of a severe X-chromosome linked disorder with the use of intracytoplasmic sperm injection are presented.
Method: A carrier of muscular dystrophy type Becker was treated with intracytoplasmic sperm injection, using spermatozoa previously stained with the Hoechst dye 33342 and sorted with flow cytometry.
Results: After transfer of one single blastocyst, an intrauterine pregnancy arose.