Publications by authors named "M Dashprakash"
Goatpox is an economically significant transboundary viral disease of goats that is caused by goatpox virus (GTPV). This study describes the prokaryotic expression of the GTPV ORF117 protein, a homologue of vaccinia virus A27L, and evaluation of its diagnostic potential in ELISA. The GTPV ORF117 gene was cloned into the pET32a vector to express recombinant ORF117 protein (rA27L) in E.
View Article and Find Full Text PDFThe study is aimed to develop and evaluate a recombinant P32 protein based ELISA for sero-monitoring and sero-surveillance using known and random/suspected serum samples for capripox infections from sheep and goats. Truncated P32 gene of goatpox virus (with an ORF of 750 bp) was expressed in E. coli BL-21 CodonPlus (DE3)-RIPL cells using pET32a vector and characterized by SDS-PAGE analysis and confirmed by western blotting as 48 kDa polyhistidine-tagged fusion protein.
View Article and Find Full Text PDFArticle Synopsis
- - The study explored the genetic relationships among twelve capripox virus isolates (sheeppox and goatpox) from India by analyzing the fusion gene (ORF 117) using PCR and sequencing techniques.
- - Sequence analysis showed a high genetic identity between goatpox and sheeppox viruses, revealing three distinct clusters in a phylogenetic tree, including one that mixed two sheeppox isolates into the goatpox group.
- - The predicted protein structure of the ORF 117 gene suggested high potential for diagnostic use due to its significant antigenic properties, making it valuable for future epidemiological studies.