Effects of endophyte-infected tall fescue, environmental temperature and prazosin injection on the rat.

Effects of an alpha 1 antagonist, prazosin, injection on the rat (Rattus rattus) exposed to warm vs normal environments and fed endophyte-infected (E+) or -free (E-) tall fescue seed were studied. Rats were injected IP daily with placebo or prazosin (1 mg/kg BW). Daily skin and rectal temperatures and food intake measurements were recorded.

Ergovaline binding and activation of D2 dopamine receptors in GH4ZR7 cells.

Ergovaline inhibition of radioligand binding to the D2 dopamine receptor and ergot alkaloid inhibition of vasoactive intestinal peptide (VIP)-stimulated cyclic AMP production in GH4ZR7 cells, stably transfected with a rat D2 dopamine receptor, were evaluated. Ergovaline inhibition of the binding of the D2-specific radioligand, [3H]YM-09151-2, exhibited a KI (inhibition constant) of 6.9 +/- 2.

The effects of fescue toxicosis on beef cattle productivity.

Consumption of tall fescue forage infested with the endophytic fungus Acremonium coenophialum can result in a condition termed "fescue toxicosis," which is characterized by decreased weight gains, milk production, conception, and serum prolactin and an inability to dissipate body heat by beef cattle. These decreases in productivity have been estimated to cost beef producers more than $600 million annually. The unthrifty appearance of cattle consuming endophyte-infected tall fescue (E+) is most evident during periods of environmental heat or cold stress, suggesting an interaction with environmental conditions.

D2 dopamine receptor response to endophyte-infected tall fescue and an antagonist in the rat.

Effects on rat brain D2 dopamine receptors by endophyte-infected tall fescue seed consumption and antagonist injection were characterized. Forty-eight male Wistar rats (225 g) in three separate trials were exposed to either 22 or 32 degrees C. Diets, to maintain similar concentrations of ergovaline, contained 10% (Trial 1) or 15% (Trials 2 and 3) endophyte-infected (E+; 325 average ppb of ergovaline) or uninfected (E-; 0 ppb of ergovaline) tall fescue seed.

Embryonic mortality in Sprague-Dawley rats induced by snakeweed (Gutierrezia microcephala).

Three experiments were conducted to determine the effects of ingested snakeweed foliage (SW) on gestating rat embryos. Mature female Sprague-Dawley rats were paired with male rats and after breeding were randomly allotted to diets containing up to 15% SW. Feeding SW caused a decrease (P < .