Robust single-molecule approach for counting autofluorescent proteins.

Using single-molecule microscopy, we present a method to quantify the number of single autofluorescent proteins when they cannot be optically resolved. This method relies on the measurement of the total intensity emitted by each aggregate until it photobleaches. This strategy overcomes the inherent problem of blinking of green fluorescent proteins.

Histologic findings in a series of 1,540 corneal allografts.

We present a series of 1,540 corneal allografts studied since 1982. Corneal edema was the major lesion in 439 corneal specimens (28.4%).

Phase I trial of recombinant interleukin-2 followed by recombinant tumor necrosis factor in patients with metastatic cancer.

In this Phase I trial, the feasibility of sequential administration of continuous intravenous recombinant interleukin-2 (rIL-2) at 18 x 10(6) IU/m2/day for 6 days, followed by three daily bolus intravenous recombinant tumor necrosis factor (rTNF) infusions at doses escalating between 10 and 120 micrograms/m2/day, was investigated in 31 patients with metastatic malignancies. Prophylactic use of indomethacin prior to and during rTNF administration was found to significantly reduce toxicity. However, despite prophylactic indomethacin, a maximum tolerated dose of rTNF of 120 micrograms/m2 was reached.

Fetal liver cell transplantation in scid mice.

Mice with severe combined immunodeficiency (CB 17 scid) received isogeneic and allogeneic fetal liver cell transplantation. Immunological reconstitution was followed by immunoglobulin production, mitogen-induced proliferation, spleen and thymus lymphoid recolonization. Scid mice injected with isogeneic fetal liver cells showed normal IgM, IgG production and subnormal Con-A induced proliferation.