Optimizing approaches for targeted integration of transgenic cassettes by integrase mediated cassette exchange in mouse and human stem cells.

To enable robust expression of transgenes in stem cells, recombinase mediated cassette exchange at safe harbour loci is frequently adopted. The choice of recombinase enzyme is a critical parameter to ensure maximum efficiency and accuracy of the integration event. We have explored the serine recombinase family of site-specific integrases and have directly compared the efficiency of PhiC31, W-beta and Bxb1 integrase for targeted transgene integration at the Gt(ROSA)26Sor locus in mouse embryonic stem cells.

Electrochemical Aptamer-Based Biosensors for Cocaine Detection in Human Saliva: Exploring Matrix Interference.

Electrochemical aptamer-based biosensors (E-aptasensors) are emerging platforms for point-of-care (POC) detection of complex biofluids. Human saliva particularly offers a noninvasive matrix and unprecedented convenience for detecting illicit drugs, such as cocaine. However, the sensitivity of cocaine E-aptasensors is significantly compromised in saliva.

Association Between Frailty and Preoperative Decision-Making in Rectal Prolapse Repair.

Introduction: Abdominal and perineal repairs for rectal prolapse are offered to patients based on surgeon assessment of risk. Interpretations of risk can vary. We sought to understand how the preoperative Risk Analysis Index (RAI) score, a validated measure of frailty, aligned with our existing decision-making process for rectal prolapse repair.

Physical and biogeochemical drivers of multi-year isoscape in the California upwelling system.

Stable isotopes of carbon (δC) and nitrogen (δN) are commonly employed to reconstruct past change in marine ecosystems and nutrient cycling. However, multiple biogeochemical and physical drivers govern spatiotemporal variability of these isotopic signals, particularly in dynamic coastal systems, complicating interpretation. Here, we coupled a modern multi-year (2010-2019) δC and δN isoscape record from intertidal mussels (Mytilus californianus) with high-resolution ocean model output and satellite chlorophyll-a observations in the California Current System (32°-43° N) to identify major drivers of isotopic variability.