Investigations on allergogenic properties of Tołpa Peat Preparation.

The ability of Tołpa Peat Preparation (TPP) to induce or enhance an allergic sensitization was tested on mice and guinea pigs. The levels of IgE antibody in the mouse sera and IgG1 as well as IgE antibody levels in guinea pig sera were evaluated by PCA (Passive Cutaneous Anaphylaxis) tests. TPP adsorbed on aluminium hydroxide gel (alum) and introduced into BALB/c mice by several subcutaneous injections was unable to stimulate the noticeable anti-TPP IgE antibody response.

The immunomodulating preparation Ukrain does not induce anaphylactic sensitization in mice and guinea pigs.

The ability of Chelidonium majus L. alkaloids derivative Ukrain to induce an anaphylactic sensitization was tested on mice and guinea pigs. The levels of IgE antibody in the mouse sera, and IgG1a, IgG1b as well as IgE antibody levels in guinea pig sera, were evaluated by passive cutaneous anaphylaxis (PCA) tests.

Histamine release from human pulmonary mast cells induced by bacterial antigens.

Antigens of four bacteria (Staphylococcus aureus, Haemophilus influenzae, Streptococcus viridans, Branhamella catarrhalis) were tested for their ability to release histamine from human pulmonary mast cells recovered by means of bronchoalveolar lavages. For the sake of comparison the action of bacterial antigens on human mesenteric and adenoidal mast cells obtained by enzymatic dispersion of the tissues was studied. BAL mast cells released histamine in response to all studied bacterial antigens.

Histamine release from human adenoidal and mesenteric mast cells induced by bacterial antigens.

The histamine-releasing capability of Staphylococcus aureus antigens was examined in human adenoidal and mesenteric mast cells obtained by enzymic dispersion of tissues from non-allergic patients. Both populations of mast cells released histamine after challenge with bacterial protein in concentrations between 5-500 micrograms/ml. The release was dependent on the dose, temperature and metabolic energy.

Histamine-releasing properties of guinea pig cardiac mast cells.

The immunization of guinea pigs with OA + Al(OH)3 induced substantial IgG1a and IgG1b antibody response and low, transient IgE response, as examined by PCA test. Cardiac mast cells obtained by enzymatic dispersion method from sensitized animals released histamine in vitro after the challenge with specific antigen (histamine release up to 21%). Cardiac mast cells obtained from nonsensitized guinea pigs were sensitive to the action of ionophore A23187 and polymyxin B only when the agents were used in high concentrations (histamine release up to 25.