In vivo effect of the organophosphorus insecticide trichlorphon on immune response of carp (Cyprinus carpio). II. Effect of high doses of trichlorphon on nonspecific immune response.

The effect of trichlorphon, one of the most widely used organophosphorus insecticides, on the nonspecific immune response in carp (Cyprinus carpio) was studied. The effect of 20,000 ppm trichlorphon on the immune response was followed for 3 and 56 days after intoxication. The effect of 10,000 ppm trichlorphon on the nonspecific immune response of carp experimentally infected by Pseudomonas alcaligenes and Aeromonas punctata was also examined.

In vivo effect of the organophosphorus insecticide trichlorphon on the immune response of carp (Cyprinus carpio). I. Effect of contamination on antibody production in relation to residue level in organs.

The organophosphorus insecticide trichlorphon was tested at medium (10 and 20 ppm) and high concentrations (20,000 ppm) on antibody production against Yersinia ruckeri vaccine. No statistically significant effect was observed on humoral response or on hematocrit. Contamination of organs was analyzed by acetylcholinesterase activity.

Effect of levamisole on the lymphocyte and macrophage activity in carp (Cyprinus carpio).

Levamisole, an anthelminthic drug which is often used in cattle, is known for its immunomodulatory effect in several species. In the present work we investigated the in vitro effects of different concentrations of levamisole on lymphocyte proliferation with mitogen in carp. At low concentrations, ie 12.

Effect of manganese ions on the immune response of carp (Cyprinus carpio) against Yersinia ruckeri.

The effect of manganese ions Mn2+ (Mn Cl2) on hematocrit and the antibody response of carp (Cyprinus carpio) was investigated after exposure of fish to contaminated water for 2.5 months. Contamination of water with 50 mg/l Mn2+ did not decreased antibody production against Yersinia ruckeri (measured by agglutination) but significantly decreased hematocrit.

Effect of pesticides (atrazine and lindane) on the replication of spring viremia of carp virus in vitro.

Atrazine and lindane are widely employed as pesticides in agriculture, which by wash off, could contaminate the aquatic environment. In this study, they were tested in vitro on the replication of the virus of Spring Viremia of Carp (SVC) in Epithelioma Papulosum Cyprini cell line. They were dissolved first in DMSO and then in medium at their solubility limit in water (28 and 10 mg/l).