Publications by authors named "M Columbaro"
Article Synopsis
- Scientists are exploring how piezoelectric nanomaterials and ultrasound can help repair tissues, and they found it might work well for creating cartilage.
- In their study, they used special tiny particles mixed in a gel and applied exact ultrasound settings, which helped certain cells turn into cartilage cells even better.
- They also created a model to predict how electricity from the ultrasound affects the materials at a tiny level, and the gel they used was safe and stuck well to cartilage, showing promise for future tests.
Article Synopsis
- Myxofibrosarcoma is a rare and aggressive soft tissue cancer that often recurs and becomes more severe with each recurrence, posing challenges for patients and clinicians alike.
- The study introduces a new cell line (MF-R 3) derived from a myxofibrosarcoma patient, which was thoroughly characterized using various biological tests to evaluate its tumor properties.
- The MF-R 3 cell line exhibits similar characteristics to the original tumor and has shown promising sensitivity to anthracycline drugs, making it a valuable model for further research and drug testing.
Osteochondral lesions, when not properly treated, may evolve into osteoarthritis (OA), especially in the elderly population, where altered joint function and quality are usual. To date, a collagen/collagen-magnesium-hydroxyapatite (Col/Col-Mg-HAp) scaffold (OC) has demonstrated good clinical results, although suboptimal subchondral bone regeneration still limits its efficacy. This study was aimed at evaluating the in vitro osteogenic potential of this scaffold, functionalized with two different strategies: the addition of Bone Morphogenetic Protein-2 (BMP-2) and the incorporation of strontium (Sr)-ion-enriched amorphous calcium phosphate (Sr-ACP) granules.
View Article and Find Full Text PDFEwing sarcoma (EWS) is a challenging pediatric cancer characterized by vast intra-tumor heterogeneity. We evaluated the RNA-binding protein IGF2BP3, whose high expression correlates with a poor prognosis and an elevated tendency of metastases, as a possible soluble mediator of inter-cellular communication in EWS. Our data demonstrate that (i) IGF2BP3 is detected in cell supernatants, and it is released inside extracellular vesicles (EVs); (ii) EVs from IGF2BP3-positive or IGF2BP3-negative EWS cells reciprocally affect cell migration but not the proliferation of EWS recipient cells; (iii) EVs derived from IGF2BP3-silenced cells have a distinct miRNA cargo profile and inhibit the PI3K/Akt pathway in recipient cells; (iv) the 11 common differentially expressed miRNAs associated with IGF2BP3-positive and IGF2BP3-negative EVs correctly group IGF2BP3-positive and IGF2BP3-negative clinical tissue specimens.
View Article and Find Full Text PDFArticle Synopsis
- * Research utilized both conventional imaging and X-ray synchrotron techniques to study mineral deposition in the SaOS-2 OS cell line, leading to a partial recovery of normal biomineralization after exposure to an osteogenic treatment for 10 days, culminating in hydroxyapatite formation.
- * During the differentiation process, mitochondrial morphology changed from elongated to rounded, suggesting a shift in energy metabolism towards increased glycolysis, which could inform new therapeutic approaches to restore normal mineralization in OS cells.