Seminal cell-free DNA and sperm characteristic's: An added biomarker for male infertility investigation.

Cell-free DNA (Cf-DNA) fragments may constitute an easy-to-measure molecular tool for guiding the choice of care provided to infertile couples who benefit assisted reproductive technology (ART) programmes. Data on Cf-DNA levels in the seminal plasma of men with sperm alterations are scarce. The objective of the present study was to quantify the presence of Cf-DNA in semen by using a quantitative real-time PCR.

Multicenter analytical performance evaluation of a fully automated anti-Müllerian hormone assay and reference interval determination.

Objectives: Anti-Müllenria hormone (AMH) is an established biomarker for assessing ovarian reserve and predicting response to controlled ovarian stimulation. Its routine clinical use is hampered by the variability and low-throughput of available enzyme-linked immunosorbent assays (ELISA). The presented study examined if a fully automated AMH electrochemiluminescence assay (ECLIA; Elecsys® AMH assay, Roche Diagnostics) was suitable for measuring AMH levels in healthy women and in those diagnosed with polycystic ovary syndrome (PCOS).

High body mass index has a deleterious effect on semen parameters except morphology: results from a large cohort study.

Objective: To evaluate the influence of body mass index (BMI) on semen characteristics.

Design: Cohort study.

Setting: Single private andrology laboratory.

Sperm deoxyribonucleic acid damage in normozoospermic men is related to age and sperm progressive motility.

Objective: To evaluate sperm DNA fragmentation in normozoospermic male partners of couples undergoing infertility evaluation.

Design: Retrospective cohort study.

Setting: Clinical andrology laboratory.