Publications by authors named "M Chopp"
Dendritic and axonal plasticity, which mediates neurobiological recovery after a stroke, critically depends on the mitochondrial function of neurons. To investigate, in vivo, neuronal mitochondrial function at the stroke recovery stage, we employed Mito-tag mice combined with cerebral cortical infection of AAV9 produced from plasmids carrying Cre-recombinase controlled by two neuronal promoters, synapsin-I (SYN1) and calmodulin-kinase IIa to induce expression of a hemagglutinin (HA)-tagged enhanced green fluorescence protein (EGFP) that localizes to mitochondrial outer membranes of SYN1 positive (SYN) and CaMKIIa positive (CaMKIIa) neurons. These mice were then subjected to permanent middle cerebral artery occlusion (MCAO) and sacrificed 14 days post stroke.
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- Recent advancements in extracellular vesicle (EV) biology are recognized for their potential impact on health and disease, particularly in vision research.
- The National Eye Institute (NEI) highlighted EV research in its 2021-2025 Strategic Plan as a key focus area within Regenerative Medicine.
- A workshop was held with twenty experts to assess the state of EV research and identify opportunities for its application in diagnosing and treating eye diseases.
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- The study investigates the role of microRNAs in Schwann cells and how their absence due to Dicer ablation leads to peripheral neuropathy in mice.
- Researchers used transgenic mice (PLP-cKO) to explore whether healthy Schwann cell-derived exosomes (SC-Exo) could alleviate nerve damage.
- Results showed that SC-Exo treatment improved nerve function and myelination in PLP-cKO mice, reversing the adverse effects of missing crucial miRNAs and proteins associated with nerve health.*
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- The study examines cerebrospinal fluid (CSF) circulation and its importance for waste clearance in the brain, specifically focusing on how CSF flow velocity relates to the glymphatic system in rats.
- Using phase-contrast MRI, researchers measured CSF flow velocity in the brain aqueduct of rats, looking at various factors such as age, gender, strain, anesthetics, and the presence of Alzheimer’s disease.
- Results showed that healthy young male Wistar rats had an average CSF flow velocity of 5.16 mm/s, while older rats and those with Alzheimer's experienced significantly reduced flow velocities.
Plexiform neurofibromas (PNs) occur in about a half of neurofibromatosis type 1 (NF1) patients and have garnered significant research attention due to their capacity for growth and potential for malignant transformation. NF1 plexiform neurofibroma (pNF1) is a complex tumor composed of Schwann cell-derived tumor cells () and the tumor microenvironment (TME). Although it has been widely demonstrated that the TME is involved in the formation of neurofibromas, little is known about the effects of the TME on the subsequent progression of human pNF1.
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