Publications by authors named "M Chipara"
Article Synopsis
- There is a growing need for effective bone disease therapies and ethical alternatives to animal research, leading to the development of long-term bone models that support osteoblast to osteocyte differentiation.
- The protocol involves seeding osteoblastic cells onto a fibrin hydrogel connected by beta-tricalcium phosphate anchors, with various analytical methods like RT-qPCR and immunofluorescence detailed for assessing the models.
- The bone models show that over 12 weeks, the cells transform the initial matrix into a collagen-rich, mineralizing structure, successfully allowing differentiation and providing a practical platform for preclinical research across various species.
Article Synopsis
- Autophagy is essential for cell survival, and its failure is linked to diseases like neurodegeneration.
- Researchers created autophagy-deficient human embryonic stem cells to study the effects of this deficiency on neuronal health.
- The study found that low levels of NAD due to increased enzyme activity cause cell death in these neurons, but boosting NAD levels can enhance cell viability, suggesting potential treatment options for related diseases.
Skin exhibits a complex structure consisting of three predominant layers (epidermis, dermis, and hypodermis). Extensive trauma may result in the loss of these structures and poor repair, in the longer term, forming scarred tissue and associated reduction in function. Although a number of skin replacements exist, there have been no solutions that recapitulate the chemical, mechanical, and biological roles that exist within native skin.
View Article and Find Full Text PDFChemical gardens are an example of a chemobrionic system that typically result in abiotic macro-, micro- and nano- material architectures, with formation driven by complex out-of-equilibrium reaction mechanisms. From a technological perspective, controlling chemobrionic processes may hold great promise for the creation of novel, compositionally diverse and ultimately, useful materials and devices. In this work, we engineer an innovative custom-built liquid exchange unit that enables us to control the formation of tubular chemical garden structures grown from the interface between calcium loaded hydrogel and phosphate solution.
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