Vitamin B6 suppresses growth and expression of albumin gene in a human hepatoma cell line HepG2.

The effect of vitamin B6 on the growth of a human hepatoma cell line HepG2 in culture was studied. The growth of HepG2 cells and protein synthesis were almost completely inhibited in medium supplemented with 5 mM pyridoxine. Pyridoxal was as effective as pyridoxine, but pyridoxamine showed no inhibitory action.

Morphological changes in rat aortic endothelial cell line stimulated by endothelin.

We have reported that endothelin (ET)-1 activates interleukin (IL)-6 production in rat aortic endothelial cell line (WAE-1 cell). In this experiment, we investigated the morphological changes induced by ET-1 in cultured WAE-1 cells. The cells were treated with ET-1 for 8 h or 24 h, and then compared with untreated cells by light and electron microscopies.

Migration of LHRH neurons derived from the olfactory placode in rats.

Using the olfactory placode of 12.5- and 14.5-day-old (E12.

Reciprocal synaptic relations between CRF-immunoreactive- and TRH-immunoreactive neurons in the paraventricular nucleus of the rat hypothalamus.

By double immunoelectron microscopy, we studied synaptic relations between corticotropin-releasing factor (CRF)-immunoreactive (ir) and thyrotropin-releasing hormone (TRH)-ir neurons in the paraventricular nucleus (PVN) of the rat hypothalamus. CRF-ir and TRH-ir neurons made reciprocal synaptic connections in the medial and periventricular parvocellular regions. These results may suggest that both the parvocellular neurons interplay on their hypophysiotropic functions within the PVN.

Somatostatin-like immunoreactive axon terminals on oxytocin-like immunoreactive neurons in the paraventricular nucleus of the rat hypothalamus.

By double label immunohistochemistry, we studied the synaptic relation between somatostatin (SS) and oxytocin (OT) or vasopressin (VP) neurons in the paraventricular nucleus (PVN) of the rat hypothalamus. In the light microscopic observation, SS-immunoreactive (ir) fibers were seen very frequently in contact with OT-ir neurons, but not with VP-ir neurons. Immunoelectron microscopic analysis revealed that somata and their dendrites of OT-ir magnocellular neurons were in synaptic contacts with SS-ir axon terminals.