Shoot Tip Cryopreservation as a Conservation Tool for Species of Quercus: Effects of Species and Environment on Recovery.

Background: The seeds of oak (Quercus) species cannot be conserved in conventional seed banks and require cryobiotechnologies for long-term, ex situ conservation.

Objective: To evaluate shoot tip cryopreservation as a method for conserving oaks ex situ.

Materials And Methods: Droplet vitrification was tested on tips from shoot cultures of four oak species; the effects of three preculture and recovery growth conditions on survival and growth were tested with Q.

Tools for the conservation of the threatened species, var. .

conservation is critical for hedging against the loss of plant diversity. For those species (exceptional species) that cannot be conserved long-term in standard seed banks, alternative methods are required, often involving culture and cryopreservation, or storage in liquid nitrogen. var.

CTC1-mediated C-strand fill-in is an essential step in telomere length maintenance.

To prevent progressive telomere shortening as a result of conventional DNA replication, new telomeric DNA must be added onto the chromosome end. The de novo DNA synthesis involves elongation of the G-rich strand of the telomere by telomerase. In human cells, the CST complex (CTC1-STN1-TEN1) also functions in telomere replication.

STN1 OB Fold Mutation Alters DNA Binding and Affects Selective Aspects of CST Function.

Mammalian CST (CTC1-STN1-TEN1) participates in multiple aspects of telomere replication and genome-wide recovery from replication stress. CST resembles Replication Protein A (RPA) in that it binds ssDNA and STN1 and TEN1 are structurally similar to RPA2 and RPA3. Conservation between CTC1 and RPA1 is less apparent.