S-Nitrosoglutathione (GSNO) is a bioactive, stable, and mobile reservoir of nitric oxide (NO), and an important player in defence responses to herbivory and pathogen attack in plants. It has been demonstrated previously that GSNO reductase (GSNOR) is the main enzyme responsible for the in vivo control of intracellular levels of GSNO. In this study, the role of S-nitrosothiols, in particular of GSNO, in systemic defence responses in Arabidopsis thaliana was investigated further.
View Article and Find Full Text PDFProtein kinase CK2 is an evolutionary conserved Ser/Thr phosphotransferase composed of two distinct subunits, alpha (catalytic) and beta (regulatory), that combine to form a tetrameric complex. Plant genomes contain multiple genes for each subunit, the expression of which gives rise to different active holoenzymes. In order to study the effects of loss of function of CK2 on plant development, we have undertaken a dominant-negative mutant approach.
View Article and Find Full Text PDFNitric oxide and S-nitrosothiols (SNOs) are widespread signaling molecules that regulate immunity in animals and plants. Levels of SNOs in vivo are controlled by nitric oxide synthesis (which in plants is achieved by different routes) and by S-nitrosoglutathione turnover, which is mainly performed by the S-nitrosoglutathione reductase (GSNOR). GSNOR is encoded by a single-copy gene in Arabidopsis (Arabidopsis thaliana; Martínez et al.
View Article and Find Full Text PDFGlutathione (GSH)-dependent formaldehyde dehydrogenase (FALDH) is a highly conserved medium-chain dehydrogenase reductase and the main enzyme that metabolizes intracellular formaldehyde in eukaryotes. It has been recently shown that it exhibits a strong S-nitrosoglutathione (GSNO) reductase activity and could be a candidate to regulate NO-signalling functions. However, there is a lack of knowledge about the tissue distribution of this enzyme in plants.
View Article and Find Full Text PDFProtein kinase CK2 is a ubiquitous Ser/Thr/Tyr kinase essential for cell viability in eukaryotes. It comprises alpha catalytic and beta regulatory subunits, which combine to form the classical tetrameric structure, alpha2beta2. Although CK2 is a component of the network that controls the eukaryotic cell cycle, very little is known about the expression patterns of genes encoding its constituent subunits, especially in plants.
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