The DnaE polymerase from Deinococcus radiodurans features RecA-dependent DNA polymerase activity.

We report in the present study on the catalytic properties of the Deinococcus radiodurans DNA polymerase III α subunit (αDr). The αDr enzyme was overexpressed in Escherichia coli, both in soluble form and as inclusion bodies. When purified from soluble protein extracts, αDr was found to be tightly associated with E.

Combined electrospray-scanning mobility particle sizer (ES-SMPS) and time-resolved synchrotron radiation-small-angle X-ray scattering (SR-SAXS) investigation of colloidal silica aggregation. Part II. Influence of aggregation initiator on gel stability.

The effect of ion specificity on the slow aggregation of silica nanoparticles with various initial morphology was investigated with an electrospray-scanning mobility particle sizer (ES-SMPS) and time-resolved synchrotron radiation-small-angle X-ray scattering (SR-SAXS). This combination provides a unique tool to monitor and resolve the early aggregate development in detail. Aggregation was induced by varying the K(2)CO(3) or KCl concentration to obtain a fixed gelation time of ∼40 min, and the results were compared with those obtained in a previous paper (Johnsson et al.

Combined electrospray-SMPS and SR-SAXS investigation of colloidal silica aggregation. Part I. Influence of starting material on gel morphology.

The slow aggregation of monodisperse, polydisperse, and preaggregated silica nanoparticles was studied with an electrospray-scanning mobility particle sizer (ES-SMPS) and time-resolved synchrotron radiation-small-angle X-ray scattering (SR-SAXS). Aggregation was induced by varying the NaCl concentration to obtain a fixed gelation time of ∼40 min. The combination of these techniques provides a unique tool to monitor and resolve the aggregate development in detail.

Determination of the Cd-bearing phases in municipal solid waste and biomass single fly ash particles using SR-microXRF spectroscopy.

By using an excitation energy of 27.0 keV, synchrotron radiation-induced micro-X-ray fluorescence (SR-microXRF) is employed to extract information regarding the composition and distribution of Cd-bearing phases in municipal solid waste (MSW) and biomass fly ashes. Significance of observation is based on statistics of totally more than 100 individual MSW and biomass fly ash particles from a fluidized bed combustion (FBC) plant.

X-ray fluorescence tomography of individual municipal solid waste and biomass fly ash particles.

Information about Cd distribution inside single municipal solid waste and biomass fly ash particles is fundamental since it affects its leachability. The internal 2D distributions of the main and trace elements in such highly inhomogeneous matrixes were successfully determined by means of the combined synchrotron radiation induced micro X-ray fluorescence (micro-SRXRF) and tomography (micro-SRXRFT) techniques. Scanning micro-SRXRF measurements show Cd elemental distribution within single fly ash particles to be inhomogeneous, but no information can be obtained about its internal distribution.