Publications by authors named "M Caceres Losa"
Arrayed CRISPR libraries extend the scope of gene-perturbation screens to non-selectable cell phenotypes. However, library generation requires assembling thousands of vectors expressing single-guide RNAs (sgRNAs). Here, by leveraging massively parallel plasmid-cloning methodology, we show that arrayed libraries can be constructed for the genome-wide ablation (19,936 plasmids) of human protein-coding genes and for their activation and epigenetic silencing (22,442 plasmids), with each plasmid encoding an array of four non-overlapping sgRNAs designed to tolerate most human DNA polymorphisms.
View Article and Find Full Text PDFBackground: Magnetic resonance imaging (MRI) has recently enabled to identify four distinct Alzheimer's disease (AD) subtypes: hippocampal sparing (HpSp), typical AD (tAD), limbic predominant (Lp), and minimal atrophy (MinAtr). To date, however, the natural history of these subtypes, especially regarding the presence of subjects switching to other MRI patterns and their clinical and biological differences, remains poorly understood.
Objective: To investigate the clinical and biological underpinnings of longitudinal atrophy pattern progression in AD.
Article Synopsis
- Aggressive pituitary neuroendocrine tumors (PitNETs) often grow despite treatment and can metastasize, making them particularly challenging to manage.
- This study analyzed tumor samples from 64 patients to investigate genetic markers, finding distinct patterns between aggressive/metastatic tumors and benign ones through genome-wide methylation and chromosomal analyses.
- The results indicate potential biomarkers that could help in identifying high-risk patients earlier, refining treatment protocols, and improving outcomes for those with aggressive pituitary tumors.
Article Synopsis
- Active acromegaly can cause serious, irreversible complications like osteopathy and fragile fractures, which are linked to longer disease duration and high growth hormone levels.
- Unlike primary osteoporosis, bone mineral density isn't a good predictor for fracture risk in acromegaly patients, as they often have normal or only slightly reduced bone mass, but compromised bone quality.
- The manuscript aims to review evidence on skeletal fragility in patients with active and resistant acromegaly, highlighting the need for disease control and potential protective effects from vitamin D and pasireotide therapies.