Publications by authors named "M C Wambulwa"

The genus contains three types of domesticates that meet various needs of ancient humans: the ornamental , the edible oil-producing , and the beverage-purposed tea plant . The genomic drivers of the functional diversification of domesticates remain unknown. Here, we present the genomic variations of 625 accessions based on a new genome assembly of var.

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Chinese Assam tea ( var. ) is an important tea crop with a long history of cultivation in Yunnan, China. Despite its potential value as a genetic resource, its genetic diversity and domestication/breeding history remain unclear.

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Multiplex polymerase chain reaction (PCR) of microsatellite loci allows for simultaneous amplification of two or more pairs of primers in a single PCR reaction; hence, it is cost and time effective. However, very few attempts have been reported in non-model species. In this study, by combining a genome-based de novo development and cross-species application approach, a multiplex PCR system comprising 5 PCR reactions of 33 microsatellites consisting of 26 novel genomic and 7 literature-sourced loci was tested for polymorphisms, cross-species transferability, and the ability to assess genetic diversity and population structure of three walnut species ( spp.

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Article Synopsis
  • Anthropogenic activities, particularly large-scale grafting of crop species onto wild plants, are leading to significant genetic diversity loss in many species, including the iron walnut (Juglans sigillata) endemic to southwestern China.
  • A study involving 149 grafted individuals from nine wild populations revealed that scions (the top part of the graft) had lower genetic diversity than their rootstocks (the base), with clear genetic separation in most populations, and instances of potential gene flow between crops and wild varieties.
  • The findings suggest that extensive grafting practices are eroding genetic diversity and disrupting the genetic structure of wild J. sigillata populations, prompting calls for conservation measures to protect natural genotypes
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Article Synopsis
  • The study investigates the genetic diversity and population structure of 12 walnut populations across Pakistan, using 31 nuclear microsatellites to assess their characteristics.
  • Findings reveal generally low genetic diversity among populations, with some exhibiting positive inbreeding coefficients, while one population in Upper Dir is notably distinct from others.
  • The research highlights key areas with high genetic diversity that need conservation and suggests implications for future walnut breeding and conservation efforts in Pakistan.
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