Utilization and integration of microalgae consortium in treating undiluted anaerobic digestate animal effluent to produce animal feed, bio-oil, and biogas.

Anaerobic digestate animal effluent (ADAE) contains high N and P nutrients which need to be treated. In this study, an integrated process was proposed using a microalgae consortium of Chlorella and Scenedesmus. The system was designed for 71 m/d (medium-sized) and 355 m/d (large-sized) animals of ADAE.

Uncovering microbiomes of the rice phyllosphere using long-read metagenomic sequencing.

The plant microbiome is crucial for plant growth, yet many important questions remain, such as the identification of specific bacterial species in plants, their genetic content, and location of these genes on chromosomes or plasmids. To gain insights into the genetic makeup of the rice-phyllosphere, we perform a metagenomic analysis using long-read sequences. Here, 1.

Bacteria can maintain rRNA operons solely on plasmids for hundreds of millions of years.

It is generally assumed that all bacteria must have at least one rRNA operon (rrn operon) on the chromosome, but some strains of the genera Aureimonas and Oecophyllibacter carry their sole rrn operon on a plasmid. However, other related strains and species have chromosomal rrn loci, suggesting that the exclusive presence of rrn operons on a plasmid is rare and unlikely to be stably maintained over long evolutionary periods. Here, we report the results of a systematic search for additional bacteria without chromosomal rrn operons.