Triplex hairpin oligosensor for ultrasensitive determination of miRNA-155 as a cancer marker using Si quantum dots and Au nanoparticles.

In this study, a new triplex hairpin oligosensor was developed for the determination of a breast cancer biomarker using silicon quantum dots (Si QD) (λ = 370 nm, λ = 482 nm) as donor and gold nanoparticles (GNP) as an acceptor in a FRET (fluorescence resonance energy transfer) mechanism. In the triplex hairpin oligosensor, a triplex-forming oligonucleotide (TFO) labeled with Si QD and a single-strand DNA labeled with GNP form a hairpin shape with a triplex structure at the hairpin stem. In a turn-on mechanism, the triplex hairpin stem is opened in the presence of sequence-specific miRNA-155 which leads to the release of the Si QD-labeled TFO probe and recovery of the fluorescence signal.

Lung T1 MRI assessments in children with mild cystic fibrosis lung disease.

Rationale: Lung T1 MRI is a potential method to assess cystic fibrosis (CF) lung disease that is safe, quick, and widely available, but there are no data in children with mild CF lung disease.

Objective: Assess the ability of lung T1 MRI to detect abnormalities in children with mild CF lung disease.

Methods: We performed T1 MRI, multiple breath washout (MBW), chest computed tomography (CT), and spirometry in a cohort of 45 children with mild CF lung disease (6-11 years of age).

Poor Oral Health Linked with Higher Risk of Alzheimer's Disease.

Alzheimer's disease (AD) is a multifactorial neurodegenerative disease characterized by cognitive and behavioral changes in older adults. Emerging evidence suggests poor oral health is associated with AD, but there is a lack of large-scale clinical studies demonstrating this link. Herein, we used the TriNetX database to generate clinical cohorts and assess the risk of AD and survival among >30 million de-identified subjects with normal oral health ( = 31,418,814) and poor oral health ( = 1,232,751).

Photothermal performance of a novel carbon dot and its conjugate with disulfiram for prostate cancer PC3 cell therapy.

To develop and employ a copper, sulfur, nitrogen-carbon quantum dot (C,S,N-CQD) multifunctional platform for synergistic cancer therapy, combining chemotherapy and photothermal treatment with cancer cell imaging. Cu,S,N-CQDs were synthesized hydrothermally, loaded with disulfiram (DSF), and characterized through UV-Vis spectrophotometry, photoluminescence, Fourier-transform infrared spectroscopy, high-resolution transmission electron microscopy, dynamic light scattering, x-ray diffraction and EDAX. Cu,S,N-CQD exhibited 5.

Optimized hybrid dielectrophoretic microchip for separation of bioparticles.

Point-of-care diagnostics requires a smart separation of particles and/or cells. In this work, the multiorifice fluid fractionation as a passive method and dielectrophoresis-based actuator as an active tool are combined to offer a new device for size-based particle separation. The main objective of the combination of these two well-established techniques is to improve the performance of the multiorifice fluid fractionation by taking advantage of dielectrophoresis-based actuator for separating particles.