Cell-type-specific roles of FOXP1 in the excitatory neuronal lineage during early neocortical murine development.

Forkhead box protein P1 (FOXP1), a transcription factor enriched in the neocortex, is associated with autism spectrum disorders (ASDs) and FOXP1 syndrome. Emx1;Foxp1 conditional deletion (Foxp1 conditional knockout [cKO]) in the mouse cortex leads to overall reduced cortex thickness, alterations in cortical lamination, and changes in the relative thickness of cortical layers. However, the developmental and cell-type-specific mechanisms underlying these changes remained unclear.

Isolation, Structure Elucidation and Biological Evaluation of Lomaiviticins F-H, Dimeric Benzofluorene Glycosides from Marine-Derived sp. Bacterium.

The discovery of new natural products remains a cornerstone of therapeutic innovation, and effective analytical tools for rapid dereplication can significantly accelerate this process. Using Isotopic Fine Structure (IFS) mass spectrometry, we rapidly identified three new dimeric benzofluorene glycosides, lomaiviticins F-H (-), from a marine-derived sp. bacterium.

A hybrid machine learning framework for functional annotation of mitochondrial glutathione transport and metabolism proteins in cancers.

Background: Alterations of metabolism, including changes in mitochondrial metabolism as well as glutathione (GSH) metabolism are a well appreciated hallmark of many cancers. Mitochondrial GSH (mGSH) transport is a poorly characterized aspect of GSH metabolism, which we investigate in the context of cancer. Existing functional annotation approaches from machine (ML) or deep learning (DL) models based only on protein sequences, were unable to annotate functions in biological contexts.

Intrathoracic pressure deviations attenuate left ventricular filling and stroke volume without pronounced myocardial mechanical alterations in healthy adults.

Intrathoracic pressure modulates cardiac loading conditions, which then influence left ventricular (LV) chamber function, and may occur with underlying myocardial mechanical alterations. We investigated the independent effects of inspiratory negative and expiratory positive intrathoracic pressure on septal geometry, LV chamber function, and rotation, twist, and strain indices. After baseline, 20 healthy adults (11M/9F, 23 ± 4 yr) performed resistive breathing to manipulate inspiratory (-30, -20, -10 cmHO) or expiratory (+10, +20 cmHO) intrathoracic pressure.