Publications by authors named "M C Ciardulli"
The advent of bioprinting has enabled the creation of precise three-dimensional (3D) cell cultures suitable for biomimetic in vitro models. In this study, we developed a novel protocol for 3D printing methacrylated collagen (ColMa, or PhotoCol®) combined with tendon stem/progenitor cells (hTSPCs) derived from human tendon explants. Although pure ColMa has not previously been proposed as a printable hydrogel, this paper outlines a robust and highly reproducible pipeline for bioprinting this material.
View Article and Find Full Text PDFA limited understanding of tendon cell biology in healthy and pathological conditions has impeded the development of effective treatments, necessitating in vitro biomimetic models for studying tendon events. We established a dynamic culture using fibrin scaffolds, bioengineered with tendon stem/progenitor cells (TSPCs) from healthy or diseased human biopsies and perfused with 20 ng/mL of human transforming growth factor-β1 for 21 days. Both cell types showed long-term viability and upregulated Scleraxis (SCX-A) and Tenomodulin (TNMD) gene expressions, indicating tenogenic activity.
View Article and Find Full Text PDFInflammation is a driving force of tendinopathy. The oxidation of phospholipids by free radicals is a consequence of inflammatory reactions and is an important indicator of tissue damage. Here, we have studied the impact of oxidized phospholipids (OxPAPC) on the function of human tenocytes.
View Article and Find Full Text PDFArticle Synopsis
- - Plastics have transformed various sectors from packaging to healthcare, but their breakdown into micro- and nanoplastics raises environmental and health concerns due to potential accumulation in nature and the human body.
- - Biodegradable plastics are being developed that can safely break down and are useful for drug delivery, but their ability to accumulate in tissues may pose toxic risks as micro- and nanoparticles.
- - The review emphasizes the need for more research on the safety of bioplastic micro- and nanoparticles, especially regarding their applications in treating brain diseases, despite their promising potential in nanomedicine.
Background: Contribution of peripheral blood mononuclear cells (PBMCs) in myogenesis is still under debate, even though blood filtration systems are commonly used in clinical practice for successfully management of critic limb ischemia.
Objectives: A commercial blood filter used for autologous PBMC transplantation procedures is characterized and used to collect PBMCs, that are then added to well-established 2D myogenic models assembled with a co-culture of bone marrow-derived mesenchymal stem cells (BM-MSCs) and skeletal myoblasts (SkMs) whit the aim of investigating their potential contribution to stem cell myogenic commitment.
Methods: A commercial blood filter was physically and chemically studied to understand its morphological characteristics and composition.