Revealing protein structures: crystallization of protein-ligand complexes - co-crystallization and crystal soaking.

Protein crystallogenesis represents a key step in X-ray crystallography studies that employ co-crystallization and ligand soaking for investigating ligand binding to proteins. Co-crystallization is a method that enables the precise determination of binding positions, although it necessitates a significant degree of optimization. The utilization of microseeding can facilitate a reduction in sample requirements and accelerate the co-crystallization process.

Long-term anatomical and functional outcomes of surgical treatment of retinal complications in children and adolescents with Stickler syndrome between 2004 and 2021.

Background: Stickler syndrome (STL) is an inherited progressive connective tissue collagen disorder. STL is the most common hereditary cause of retinal complications, retinal tears, and the development of retinal detachment (RD) in childhood. The aim of the study was to evaluate the long-term anatomical and functional results of surgical treatment of retinal complications in children and adolescents affected by STL.

Post-traumatic exogenous endophthalmitis caused by Nocardia farcinica.

A case report of post-traumatic exogenous endophthalmitis caused by Nocardia farcinica, including treatment procedures, microbiology examination, and systemic medications. A 23-year-old male suffered a penetrating corneal injury that was treated with sutures. On the thirteenth day after the final suture was removed, an anterior uveitis developed and progressed to whitish, plump, nodular, and tufted exudates within the anterior chamber over the next 10 days; this led to an indication for intraocular surgery.

Higher Total Insulin Dose Has Positive Effect on Corneal Nerve Fibers in DM1 Patients.

Purpose: Neuropathies are among the most common long-term complications of diabetes mellitus (DM) and good glycemic control is essential in prevention of this complication. DM patients with similar mean glucose levels or HbA1c levels often exhibit differences in glucose variability. We tested for possible associations between parameters of glycemia compensation and corneal sub-basal nerve fiber status.

[Correlation of intraocular pressure measured by applanation tonometry, noncontact tonometry and TonoPen with central corneal thickness].

Unlabelled: The purpose of the study was to determine the dependence of intraocular pressure (IOP) measured by Goldmann applanation tonometer (AT), non-contact tonometer (BT) and Tone-Pen (TP) on central corneal thickness (CCT) as well as the respective comparison in relation to the central corneal thickness.

Methods: CCT and IOP were assessed in 106 eyes of 106 subjects aged 7-77 years (mean 34.3 +/- 17.