Publications by authors named "M Burday"
Article Synopsis
- The research focuses on developing a rapid detection assay for a tier 1 select agent that can cause severe disease, which is crucial for responding to bioterrorism threats.
- The assay uses a filter-based cartridge to process blood samples and performs PCR amplification, achieving an impressive limit of detection of 5 genome equivalents and 10 CFU/ml of blood.
- Results show the assay is highly specific (100% for non-anthrax samples) and can deliver results in under 90 minutes, potentially facilitating quicker treatment in emergency situations.
Background: Rapid detection of bloodstream infections (BSIs) can be lifesaving. We investigated the sample processing and assay parameters necessary for highly-sensitive detection of bloodstream bacteria, using Staphylococcus aureus as a model pathogen and an automated fluidic sample processing-polymerase chain reaction (PCR) platform as a model diagnostic system.
Methodology/principal Findings: We compared a short 128 bp amplicon hemi-nested PCR and a relatively shorter 79 bp amplicon nested PCR targeting the S.
A real-time PCR assay with the ability to rapidly identify all pathogenic bacteria would have widespread medical utility. Current real-time PCR technologies cannot accomplish this task due to severe limitations in multiplexing ability. To this end, we developed a new assay system which supports very high degrees of multiplexing.
View Article and Find Full Text PDFCurrent nucleic acid amplification methods to detect Mycobacterium tuberculosis are complex, labor-intensive, and technically challenging. We developed and performed the first analysis of the Cepheid Gene Xpert System's MTB/RIF assay, an integrated hands-free sputum-processing and real-time PCR system with rapid on-demand, near-patient technology, to simultaneously detect M. tuberculosis and rifampin resistance.
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