Influence of some mineral ions on lipid peroxidation in vitro.

Recently, a growing interest has been recorded in mineral content of mammalian diet, which might impair their development. Focused on the topic, we studied the effect of Al3+, Si4+, Sr2+ and Na2S on the intensity of malondialdehyde (MDA) production in vitro. MDA, as one of oxidative stress markers, was determined in rat brain homogenates in the conditions of lipid peroxidation (LP) activated by iron ions and ascorbate.

Effect of mineral water from Trenčianske Teplice (drinkable source, drill SB-3) on lipid peroxidation in vitro.

Influence of mineral water from Trenčianske Teplice (drinkable source) on lipid peroxidation processes was determined in model situations under in vitro conditions using the brain tissue. The central nervous system was selected because it is especially sensitive to the radical-induced damage. In addition, there is a high content of polyunsaturated fatty acids in the brain which has a low antioxidant capacity and is relatively rich in iron ions--enhancers of lipid peroxidation processes.

K(+)-p-nitrophenylphosphatase activity in rat brain and liver.

K(+)-p-nitrophenylphosphatase (K(+)pNPPase) is the enzyme, which is considered to be involved in K(+)-dependent hydrolysis of the phosphoenzyme in the reaction cycle of Na(+), K(+)ATPase. The aim of our present study was to characterize some features of K(+)pNPPase in homogenates of the rat brain and liver. We determined p-nitrophenylphosphatase (pNPPase) activity in the presence of various ion combinations (Mg(2+)+ K(+), Mg(2+), K(+)).