Publications by authors named "M Bomsel"
Erratum for Barbosa Bomfim et al., "CGRP inhibits SARS-CoV-2 infection of bronchial epithelial cells, and its pulmonary levels correlate with viral clearance in critical COVID-19 patients".
J Virol
December 2024
Despite an undetectable plasma viral load as a result of antiretroviral therapy, HIV-1-infected individuals with poor immune reconstitution harbor infectious HIV-1 within their platelets. Megakaryocytes, as platelet precursors, are the likely cellular origin of these HIV-1-containing platelets. To investigate the mechanisms that allow megakaryocytes to support HIV-1 infection, we established in vitro models of viral infection using hematopoietic stem cell-derived megakaryocytes and the megakaryocytic MEG-01 cell line.
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- - The study examines the role of calcitonin gene-related peptide (CGRP) in critical COVID-19 patients, finding that levels of CGRP are elevated in both the plasma and lungs during the early stages of infection with SARS-CoV-2.
- - CGRP and its stable analog, SAX, were found to inhibit the infection of bronchial cells by SARS-CoV-2 variants in a dose-dependent manner, suggesting that CGRP plays a protective role against the virus.
- - The research indicates that increased CGRP levels may help in viral clearance by directly inhibiting SARS-CoV-2 replication, which opens potential avenues for CGRP-based therapies in managing COVID-19.
Low and persistent levels of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA/protein/virus can be detected in clinical samples months after infection, possibly related to the emergence of SARS-CoV-2 variants or development of long coronavirus disease. Here, we present a protocol to detect low levels of viral RNA together with protein using flow cytometry and microscopy. We describe steps for cell infection with SARS-CoV-2 and quantification by fluorescence in situ hybridization-flow cytometry.
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