Publications by authors named "M Bhakat"

Sperm motility is the prime functional attribute for semen quality and fertility of the bull. However, the bull's age directly affects the semen quality, and the bull's fertility and productive life decline with age. Even though research on age has been conducted in the past, it is still unclear how old a bull should be maintained at artificial insemination centers.

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Bull fertility is a multi-factorial trait and is affected by many factors, such as nutrition, genetics, and epigenetics. Superior quality male germplasm with high genetic merit helps to improve the livestock production trait. To achieve the target of livestock production, the availability of superior male germplasm is a great concern.

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Article Synopsis
  • - The study investigates the role of miRNAs in the seminal plasma extracellular vesicles (SP-EVs) from high-fertile (HF) and low-fertile (LF) Sahiwal bulls, focusing on how these molecules might affect fertility through genetic and molecular signaling.
  • - SP-EVs were isolated and analyzed, showing different sizes and particle concentrations between HF and LF bulls, but the overall differences in size and concentration were not statistically significant.
  • - High-throughput miRNA sequencing identified 310 miRNAs, with significant differences in expression levels between HF and LF bulls, particularly highlighting bta-miR-195, which was found to be highly expressed in HF spermatozoa and may be linked to improved fertility
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Mastitis is a global concern in the dairy sector, demanding innovative solutions for effective management for quality lifetime milk production. In this study, infrared thermography (IRT) as a non-invasive technology was integrated into routine farm activities for continuous health monitoring of animals. For 30 days, we systematically monitored the udder health status in 40 Sahiwal cows (160 quarters), employing IRT along with the California Mastitis Test (CMT).

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Backgroud: Before fertilization, spermatozoa undergo a crucial maturation step called capacitation, which is a unique event regulates the sperm's ability for successful fertilization. The capacitation process takes place as the spermatozoa pass through the female reproductive tract (FRT). Dihydrolipoamide dehydrogenase (DLD) protein is a post-pyruvate metabolic enzyme, exhibiting reactive oxygen species (ROS) production which causes capacitation.

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