Monoclonal antibodies protect against respiratory syncytial virus infection in mice.

Twenty-five monoclonal antibodies (Mab) to respiratory syncytial virus (RSV) and two to hepatitis B virus were inoculated intravenously into mice. Twenty-four hours later the mice were challenged intranasally with RSV. Eleven of 14 Mab against fusion protein and four out of six Mab against a larger glycoprotein (GP84) significantly reduced the titre of RSV in the lungs when mice were killed 5 days later.

Preparation and properties of monoclonal antibodies to the anabolic agent zeranol.

Monoclonal antibodies have been produced using two haptens, zeranol-7-hemisuccinate coupled to bovine serum albumin and zeranol-16-carboxypropyl ether coupled to human serum albumin. An assessment of cross-reactivity demonstrated that the monoclonal antibody raised against the 7-hemisuccinate derivative reacted with zeranol (100%), talernol (12%), zearalenone (17%), zearalanone (100%) and alpha- and beta-zearalenol (17% and less than 0.01%, respectively).

The characterization and uses of monoclonal antibodies to respiratory syncytial virus.

Fifteen hybridomas secreting monoclonal antibodies against respiratory syncytial virus (RSV) have been produced. Three react with nucleoprotein (MW 42 000 daltons), 1 with phosphoprotein (MW 36 000 daltons), 1 with a larger protein (MW 60 000 daltons) and 10 with the fusion glycoprotein (MW 46 000 + 22 000 daltons). By immunofluorescent staining of infected cells, 5 monoclonal antibodies bind to cytoplasmic inclusions and the remainder give diffuse cytoplasmic fluorescence.

Studies on cross protection induced in calves by rotaviruses of calves, children and foals.

Inoculation at birth with a live attenuated strain of a bovine rotavirus isolated in the USA (scourvax-reo) induced protection in five gnotobiotic calves seven to 21 days later against a UK isolate of pathogenic bovine rotavirus. However, no protection was induced in three calves challenged three to five days after vaccination. There was a close antigenic relationship demonstrated between the two bovine rotavirus isolates.