Two species from the families Acipenseridae and Polyodontidae, Russian sturgeon (, Brandt and Ratzeberg, 1833; functional tetraploid) and American paddlefish (, Walbaum 1792, functional diploid) were hybridized. The hybridization was repeated using eggs from three sturgeon and sperm from four paddlefish individuals. Survival in all hybrid family groups ranged from 62% to 74% 30 days after hatching.
View Article and Find Full Text PDFLocal extreme climatic conditions occurring as a result of global climate change may interfere with the reproduction of animals. In the present study fish spermatozoa were incubated at different temperatures (20, 25, 30 and 40 °C) for 10 and 30 minutes, respectively and plasma membrane integrity and mitochondrial membrane potential changes were evaluated with flow cytometry using SYBR-14/PI and Mitotracker Deep Red FM fluorescent dyes. No significant differences were found in plasma membrane integrity at either incubation temperatures or time points.
View Article and Find Full Text PDFThe aim of the present pilot study was to apply a flow cytometric assay, the so-called OxyDNA test, to determine the level of oxidative DNA damage in fish spermatozoa exposed to different concentrations (0.01-10,000 mg/L) of cadmium. Milt was collected from three randomly selected Prussian carp (Carassius auratus gibelio) males.
View Article and Find Full Text PDFTo understand the molecular evolution of mitochondrial genomes (mitogenomes) in the genus Odontobutis, the mitogenome of Odontobutis yaluensis was sequenced and compared with those of another four Odontobutis species. Our results displayed similar mitogenome features among species in genome organization, base composition, codon usage, and gene rearrangement. The identical gene rearrangement of trnS-trnL-trnH tRNA cluster observed in mitogenomes of these five closely related freshwater sleepers suggests that this unique gene order is conserved within Odontobutis.
View Article and Find Full Text PDFMitochondrial DNA A DNA Mapp Seq Anal
September 2016
The complete mitochondrial genome of Sander lucioperca has been sequenced and analyzed in this study. It was a circular double-stranded DNA molecule of 16,541 base pairs (bp) in length and exhibited 37 typical animal mitochondrial genes. The gene order and base composition were similar to those of other percid species.
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