Publications by authors named "M Benhaim"

Article Synopsis
  • Influenza hemagglutinin (HA) is crucial for the virus's entry as it helps with receptor binding and fusion of membranes, with clear structures observed at two stages: prefusion and postfusion.
  • Research has started to uncover the intermediate stages of HA during the fusion process, which aids in understanding its activation and how it changes shape to complete fusion.
  • This study utilized hydrogen-deuterium exchange mass spectrometry to compare HA subtypes H1 and H3, revealing distinct behaviors in their structural changes during fusion, especially when influenced by a particular antibody.
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Background: Pyogenic liver abscess (PLA) is an uncommon but potentially life-threatening condition. In recent years, advances in diagnostics and management have led to early diagnosis and treatment and decreased mortality. We present recent data from a large series of patients with PLA and examine the trends in the management of PLA over a period of 50 years.

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Purpose: To examine the ex- vivo ability of explanted human tumors and normal tissue to activate liposomal mitomycin C lipidic prodrug (MLP) by releasing the active free drug form, mitomycin C (MMC).

Methods: We tested conversion of MLP to MMC in an ex vivo assay using explanted tissues obtained during routine surgery to remove primary tumors or metastases. Tumor and adjacent normal tissue were obtained from freshly explanted tumors and were immediately deep frozen at - 70 °C.

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Article Synopsis
  • The envelope glycoprotein (Env) is crucial for HIV's ability to evade the immune system and is highly variable among different strains of the virus.
  • Research using hydrogen/deuterium-exchange mass spectrometry (HDX-MS) indicates that the dynamics and structural flexibility of Env differ significantly across various HIV isolates.
  • These differences affect neutralization sensitivity, receptor activation, and the stability of the Env trimer, with certain mutations stabilizing some isolates but having minimal impact on others.
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HIV-1 Env mediates viral entry into host cells and is the sole target for neutralizing antibodies. However, Env structure and organization in its native virion context has eluded detailed characterization. Here, we used cryo-electron tomography to analyze Env in mature and immature HIV-1 particles.

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