Publications by authors named "M Benassy"
Article Synopsis
- Understanding RNA targeting to membraneless organelles like P-bodies (PBs) is crucial for revealing their functions in cells.
- This study shows that PBs in HEK293 cells undergo significant changes in RNA content throughout the cell cycle, with different mRNA localization patterns appearing at various stages (G1, S, G2).
- The findings suggest that PBs actively sort mRNAs based on their translation status and characteristics, implying that they play a more dynamic role than merely housing excess untranslated mRNAs.
The localization of RNAs in cells is critical for many cellular processes. Whereas motor-driven transport of ribonucleoprotein (RNP) condensates plays a prominent role in RNA localization in cells, their study remains limited by the scarcity of available tools allowing to manipulate condensates in a spatial manner. To fill this gap, we reconstitute in cellula a minimal RNP transport system based on bioengineered condensates, which were functionalized with kinesins and dynein-like motors, allowing for their positioning at either the cell periphery or centrosomes.
View Article and Find Full Text PDFArticle Synopsis
- The study explores the relationship between mRNA translation and decay, revealing that this interplay is not fully understood.
- By analyzing various transcriptomes, it was found that mRNA content (specifically GC vs. AU richness) significantly influences mRNA localization, translation efficiency, and stability.
- The findings indicate that AU-rich mRNAs are less efficiently translated and follow different decay pathways compared to GC-rich mRNAs, highlighting a complex system of post-transcriptional regulation in human cells.
Recovery from traumatic spinal cord injury (SCI) usually fails due to a cascade of cellular and molecular events that compromise neural tissue reconstitution by giving rise to glial scarring and cavity formation. We designed a scaffold material for SCI treatment containing only chitosan and water as fragmented physical hydrogel suspension (Chitosan-FPHS), with defined degree of acetylation (DA), polymer concentration, and mean fragment size. Implantation of Chitosan-FPHS alone into rat spinal cord immediately after a bilateral dorsal hemisection promoted reconstitution of spinal tissue and vasculature, and diminished fibrous glial scarring: with astrocyte processes primarily oriented towards the lesion, the border between lesion site and intact tissue became permissive for regrowth of numerous axons into, and for some even beyond the lesion site.
View Article and Find Full Text PDFThe microtubule-associated protein MAP1B plays a key role in axon regeneration. We investigated the role of GSK3-mediated MAP1B phosphorylation in local fine-tuning of neurite branching and the underlying microtubule (MT) dynamics. In wildtype adult dorsal root ganglia (DRG) neurons, MAP1B phosphorylation is locally reduced at branching points, and branching dynamics from growth cones and distal neurite shafts is increased upon GSK3 inhibition.
View Article and Find Full Text PDF