Gas chromatographic quantification of aliphatic aldehydes in freshly distilled Calvados and Cognac using 3-methylbenzothiazolin-2-one hydrazone as derivative agent.

A new precise and sensitive method was used for the quantification of aliphatic aldehydes from C5 to C11 in highly ethanolic beverages such as freshly distilled spirits. Carbonyl compounds were derivatized using 3-methylbenzothiazolin-2-one hydrazone (MBTH) and then separated and detected by gas chromatography-mass spectrometry (GC-MS). Selective mass spectrometric detection of molecular ions of derivatives was performed to obtain a good sensibility (0.

[Evaluation of lipid peroxidation by measuring thiobarbituric acid reactive substances].

Malondialdehyde assay is the most generally used test in the appreciation of the role of oxidative stress in disease. Malondialdehyde is one of several products formed during the radical induced decomposition of polyunsaturated fatty acids. Most often, malondialdehyde assay used its reactivity at high temperature and low pH, towards thiobarbituric acid.

[Blood ethanol assay by gas chromatography].

Blood ethanol measurement can be performed by gas chromatography if the samples are diluted before tungstate-sulfuric acid deproteinization. A good knowledge of linearity area is needed for to obtain with this method the same results than with the oxydimetric method.

Subpicogram determination of malondialdehyde by gas-liquid chromatography with nitrogen phosphorus detector. I. Standardization.

A method for the measurement of malondialdehyde (MDA) using GLC with a nitrogen phosphorus detector (GLC/NPD) is described and evaluated. The method uses 2-hydrazinobenzothiazole (HBT) which forms condensation derivatives with MDA, acetoacetaldehyde, and acetylacetone (AA). GC/MS and 13C NMR studies of the three derivatives obtained showed that they are 2-(pyrazol-1'-yl)benzothiazoles and that they can be separated by GLC/NPD.