Antiphospholipid and antioangiogenic activity in females with recurrent miscarriage and antiphospholipid syndrome.

Background Antiphospholipid syndrome is an autoimmune disease characterized by thrombosis, fetal losses and thrombocytopenia associated to antiphospholipid antibodies. They are directed to phospholipids, such as cardiolipins (anticardiolipin) and lupus anticoagulant or to complexes formed by phospholipids and protein cofactors, such as β2 glycoprotein 1 (a-β2GP1) and annexin V (a-annexin V). These auto-antibodies may be considered as a family of antibodies involved in thrombotic events and antiphospholipid activity.

A test field for the second-order advantage in bilinear least-squares and parallel factor analyses: fluorescence determination of ciprofloxacin in human urine.

The analytical performances of two algorithms, the recently introduced bilinear least-squares (BLLS) and the popular parallel factor analysis (PARAFAC), are compared as regards second-order fluorescence data recorded for the determination of the fluoroquinolone antibiotic ciprofloxacin in human urine samples. The applied chemometric methodologies employ different strategies for exploiting the so-called second-order advantage, which allows one to obtain individual concentrations of calibrated analytes in the presence of any number of uncalibrated (urine) components. Analysis of a spiked urine test set (in the analyte concentration range 0-200 mg L(-1)) showed that BLLS provides results of slightly better quality than PARAFAC.

A validated spectrofluorometric method for the determination of celecoxib in capsules.

A rapid, selective, sensitive and simple fluorescence method was developed for the direct determination of celecoxib in capsules. The capsules were emptied, pulverized and dissolved in either ethanol or acetonitrile, sonicated and filtered. Direct fluorescence emission was measured at 355+/-5 nm (exciting at 272 nm).

Spectrofluorometric determination of naproxen in tablets.

A rapid, selective, sensitive and simple fluorescence method was developed for the direct determination of naproxen in tablets. The tablets were triturated, dissolved in either NH(3) or NaOH solution, sonicated, filtered and then direct fluorescence emission was read at 353 nm (exciting at 271 nm). In order to validate the method the results were compared with those obtained by the USP XXIV NF 19 Pharmacopeia reference method (high performance liquid chromatography).

Heterologous exchanges of the glycoprotein and the matrix protein in a Novirhabdovirus.

Infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicemia virus (VHSV) are two salmonid rhabdoviruses replicating at low temperatures (14 to 20 degrees C). Both viruses belong to the Novirhabdovirus genus, but they are only distantly related and do not cross antigenically. By using a recently developed reverse-genetic system based on IHNV (S.