[Not Available].

Introduction: Le niveau d'expression des molécules HLA-DR à la surface des monocytes (mHLA-DR) est un marqueur diagnostique utilisé pour évaluer l'immunité des patients en réanimation (choc septique, polytraumatisés, brulures, greffe et plus récemment Covid-19). Il est également utilisé comme un outil de stratification dans les essais cliniques utilisant des thérapies immunostimulantes chez ces patients. L'objectif de cette étude était d'évaluer les performances analytiques d'une méthode de cytométrie en flux pour mesurer mHLA-DR afin de répondre aux exigences de la norme NF EN ISO 15189 dans le cadre de l'accréditation des laboratoires de biologie médicale.

Quantification of Legionella DNA certified reference material by digital droplet PCR.

A value was assigned in 2009 to the Legionella DNA Certified Reference Material, and the stability study conducted using quantitative PCR found a low level of degradation. Herein, the Digital Droplet PCR method for Legionella DNA was qualified and used to provide absolute quantification of the CRM.

Evaluation of the Accelerate Pheno™ system for rapid identification and antimicrobial susceptibility testing of Gram-negative bacteria in bloodstream infections.

Identification and antimicrobial susceptibility testing (AST) are critical steps in the management of bloodstream infections. Our objective was to evaluate the performance of the Accelerate Pheno™ System, CE v1.2 software, for identification and AST of Gram-negative pathogens from positive blood culture bottles.

[Towards the development of the orthogeriatric care model].

The frequency and seriousness of fractures in frail elderly people has led to the creation of an orthogeriatric service in Paris, between Ambroise-Paré and Sainte-Périne hospitals. This care model, focused on early postoperative geriatric follow-up and rehabilitation care, has resulted in an increase in the flow of trauma patients admitted to Sainte-Périne, reduced mortality and higher home-discharge rate.

The characterization and certification of a quantitative reference material for Legionella detection and quantification by qPCR.

Aims: The characterization and certification of a Legionella DNA quantitative reference material as a primary measurement standard for Legionella qPCR.

Methods And Results: Twelve laboratories participated in a collaborative certification campaign. A candidate reference DNA material was analysed through PCR-based limiting dilution assays (LDAs).