Publications by authors named "M Barcella"
Introduction: Early intervention represents an opportunity to contain psychological distress and intervene promptly on conditions that, otherwise, could assume a chronic course. Based on these observations, an early intervention programme for people 18-25 years old, the 'Progetto Giovani' (Youth Project), was implemented in two adult mental health services (AMHSs) in the northwest of Italy.
Methods: This study aims to describe the socio-demographic and clinical characteristics of the patients included in the Youth Project from 1 January 2013 to 31 December 2022.
Ex vivo activation is a prerequisite to reaching adequate levels of gene editing by homology-directed repair (HDR) for hematopoietic stem and progenitor cell (HSPC)-based clinical applications. Here, we show that shortening culture time mitigates the p53-mediated DNA damage response to CRISPR-Cas9-induced DNA double-strand breaks, enhancing the reconstitution capacity of edited HSPCs. However, this results in lower HDR efficiency, rendering ex vivo culture necessary yet detrimental.
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- The study evaluates "Functional Management and Recovery," a psychoeducational intervention tailored for individuals with psychotic disorders in psychiatric residential facilities (PRFs) in Italy, aiming to enhance recovery and functioning due to the lack of structured interventions in these settings.
- A total of 66 participants underwent a multi-center study with pre-treatment and six-month follow-up evaluations, using various scales to measure social functioning, psychopathological status, recovery, cognitive functioning, stress management, emotional intelligence, and user satisfaction.
- Results showed significant improvements in multiple areas, with 63 participants completing the study, highlighting a predominance of schizophrenia (82.5%) and addressing the need for effective recovery strategies in PRFs.
Hematopoietic stem cell gene therapy (GT) using a γ-retroviral vector (γ-RV) is an effective treatment for Severe Combined Immunodeficiency due to Adenosine Deaminase deficiency. Here, we describe a case of GT-related T-cell acute lymphoblastic leukemia (T-ALL) that developed 4.7 years after treatment.
View Article and Find Full Text PDFIn physiological conditions, few circulating hematopoietic stem/progenitor cells (cHSPCs) are present in the peripheral blood, but their contribution to human hematopoiesis remain unsolved. By integrating advanced immunophenotyping, single-cell transcriptional and functional profiling, and integration site (IS) clonal tracking, we unveiled the biological properties and the transcriptional features of human cHSPC subpopulations in relationship to their bone marrow (BM) counterpart. We found that cHSPCs reduced in cell count over aging and are enriched for primitive, lymphoid, and erythroid subpopulations, showing preactivated transcriptional and functional state.
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