Targeted Prostate Biopsy: How, When, and Why? A Systematic Review.

Objective: Prostate cancer, the second most diagnosed cancer among men, requires precise diagnostic techniques to ensure effective treatment. This review explores the technological advancements, optimal application conditions, and benefits of targeted prostate biopsies facilitated by multiparametric magnetic resonance imaging (mpMRI).

Methods: A systematic literature review was conducted to compare traditional 12-core systematic biopsies guided by transrectal ultrasound with targeted biopsy techniques using mpMRI.

Broadband serrodyne phase modulation for optical frequency standards and spectral purity transfer.

We perform low phase noise, efficient serrodyne modulation for optical frequency control and spectral purity transfer between two ultrastable lasers. After characterizing serrodyne modulation efficiency and its bandwidth, we estimate the phase noise induced by the modulation setup by developing a novel, to the best of our knowledge, composite self-heterodyne interferometer. Exploiting serrodyne modulation, we phase locked a 698 nm ultrastable laser to a superior ultrastable laser source at 1156 nm by means of a frequency comb as a transfer oscillator.

Cultural practices of health care and illness of elderly people descendants of Ukrainians.

Objective: To interpret the cultural practices of health care and illness of elderly people descendants of Ukrainians.

Method: Qualitative research developed with two family general informants and 22 elderly key informants, from August 2017 to March 2020, in a rural community, analyzed through domains, taxonomies and cultural theme. The cultural theme is based on the Theory of Culture Care Diversity and Universality.

Handgrip strength in older adults and driving aptitude.

Objective: to analyze handgrip strength as a predictor of the inability to drive in older adults.

Method: a cross-sectional study conducted in traffic clinics with 421 older adults in Curitiba-Paraná from January 2015 to December 2018. A sociodemographic and clinical questionnaire, handgrip strength test, and queries from the National Registry of Qualified Drivers form were applied.

Cell cycle-dependent binding between Cyclin B1 and Cdk1 revealed by time-resolved fluorescence correlation spectroscopy.

Measuring the dynamics with which the regulatory complexes assemble and disassemble is a crucial barrier to our understanding of how the cell cycle is controlled that until now has been difficult to address. This considerable gap in our understanding is due to the difficulty of reconciling biochemical assays with single cell-based techniques, but recent advances in microscopy and gene editing techniques now enable the measurement of the kinetics of protein-protein interaction in living cells. Here, we apply fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy to study the dynamics of the cell cycle machinery, beginning with Cyclin B1 and its binding to its partner kinase Cdk1 that together form the major mitotic kinase.