Sodium Energetic Cycle in the Natronophilic Bacterium .

As inhabitants of soda lakes, are halo- and alkaliphilic bacteria that have previously been shown to respire with the first demonstrated Na-translocating cytochrome- oxidase (CO). The enzyme generates a sodium-motive force (Δ) as high as -270 mV across the bacterial plasma membrane. However, in these bacteria, operation of the possible Δ consumers has not been proven.

G-quadruplex-forming oligodeoxyribonucleotides activate leukotriene synthesis in human neutrophils.

Human polymorphonuclear leukocytes (PMNLs, neutrophils) play a major role in the immune response to bacterial and fungal infections and eliminate pathogens through phagocytosis. During phagocytosis of microorganisms, the 5-lipoxygenase (5-LOX) pathway is activated resulting in generation of leukotrienes, which mediate host defense. In this study, a library of oligodeoxyribonucleotides (ODNs) with varying numbers of human telomeric repeats (d(TTAGGG)) and their analogues with phosphorothioate internucleotide linkages and single-nucleotide substitutions was designed.

Dissulfurirhabdus thermomarina gen. nov., sp. nov., a thermophilic, autotrophic, sulfite-reducing and disproportionating deltaproteobacterium isolated from a shallow-sea hydrothermal vent.

A thermophilic, anaerobic, chemolithoautotrophic bacterium, strain SH388, was isolated from a shallow, submarine hydrothermal vent (Kuril Islands, Russia). Cells of strain SH388 were Gram-stain-negative short rods, 0.2-0.

Inmirania thermothiophila gen. nov., sp. nov., a thermophilic, facultatively autotrophic, sulfur-oxidizing gammaproteobacterium isolated from a shallow-sea hydrothermal vent.

A novel thermophilic, facultatively autotrophic bacterium, strain S2479T, was isolated from a thermal spring located in a tidal zone of a geothermally heated beach (Kuril Islands, Russia). Cells of strain S2479T were rod-shaped and motile with a Gram-negative cell-wall type. The temperature range for growth was 35-68 °C (optimum 65 °C), and the pH range for growth was pH 5.

Chromatographic isolation of the functionally active MutS protein covalently linked to deoxyribonucleic acid.

DNA metabolism is based on formation of different DNA-protein complexes which can adopt various conformations. To characterize functioning of such complexes, one needs a solution-based technique which allows fixing a complex in a certain transient conformation. The crosslinking approach is a popular tool for such studies.