Publications by authors named "M B Rookmaaker"
Tubuloids are advanced in vitro models obtained from adult human or mouse kidney cells with great potential for modelling kidney function in health and disease. Here, we developed a polarized human and mouse tubuloid epithelium on cell culture inserts, namely Transwell™ filters, as a model of the distal nephron with an accessible apical and basolateral side that allow for characterization of epithelial properties such as leak-tightness and epithelial resistance. Tubuloids formed a leak-tight and confluent epithelium on Transwells™ and the human tubuloids were differentiated towards the distal part of the nephron.
View Article and Find Full Text PDFArticle Synopsis
- Nuclear receptors (NRs) are important transcription factors in the small intestine that respond to various signals and influence multiple biological processes such as nutrient absorption and drug metabolism.
- This study examines the activation of six different NRs using small molecule agonists in human intestinal organoids, highlighting their roles in lipid, glucose, and amino acid absorption, as well as electrolyte balance.
- Unique target genes associated with specific NRs were identified, providing valuable insights that could lead to enhanced pharmacological and therapeutic applications.
Estimating glomerular filtration (eGFR) after Continuous Renal Replacement Therapy (CRRT) is important to guide drug dosing and to assess the need to re-initiate CRRT. Standard eGFR equations cannot be applied as these patients neither have steady-state serum creatinine concentration nor average muscle mass. In this study we evaluate the combination of dynamic renal function with CT-scan based correction for aberrant muscle mass to estimate renal function immediately after CRRT cessation.
View Article and Find Full Text PDFArticle Synopsis
- Glomerular filtration rate (GFR) is an important indicator of kidney function, commonly estimated using formulas based on factors like serum creatinine, age, and sex.
- The estimated GFR (eGFR) is limited as it doesn't capture the full range of kidney functions and should be used alongside urine analysis and other clinical information for a thorough assessment of kidney health.
- For effective monitoring, factors such as medication, diabetes, obesity, and pregnancy need to be carefully considered alongside eGFR to understand the overall impact on kidney function for the patient.