Evaluating the implementation of the rapid prenatal exome sequencing (pES) service in England.

Introduction In October 2020, a national rapid prenatal exome sequencing (pES) service was rolled out across the English National Health Service (NHS). This service is delivered by multiple clinical and two laboratory teams. Whilst there was high level national guidance to support implementation, it was unclear how the service has been delivered in practice.

WNT signaling contributes to the extrahepatic bile duct proliferative response to obstruction in mice.

Biliary obstruction and cholangiocyte hyperproliferation are important features of cholangiopathies affecting the large extrahepatic bile duct (EHBD). The mechanisms underlying obstruction-induced cholangiocyte proliferation in the EHBD remain poorly understood. Developmental pathways, including WNT signaling, are implicated in regulating injury responses in many tissues, including the liver.

Allosteric substrate release by a sialic acid TRAP transporter substrate binding protein.

The tripartite ATP-independent periplasmic (TRAP) transporters enable Vibrio cholerae and Haemophilus influenzae to acquire sialic acid, aiding their colonization of human hosts. This process depends on SiaP, a substrate-binding protein (SBP) that captures and delivers sialic acid to the transporter. We identified 11 nanobodies that bind specifically to the SiaP proteins from H.

Implementation of a National Prenatal Exome Sequencing Service in England: Cost-Effectiveness Analysis.

Objective: Prenatal exome sequencing (pES) for diagnosing fetal structural anomalies commenced in the English National Health Service (NHS) in 2020. We evaluated cost-effectiveness to the healthcare system, and costs to families, of pES in addition to standard testing, compared to standard testing alone.

Design: A cost-effectiveness analysis combining costs, outcomes, parent and professional interview and professional survey data.

Thyroid hormones activate TH/E receptor/regulator system and drive Na/K-ATPase in the ovarian wall of hypothyroid air-breathing fish (Anabas testudineus Bloch).

In fish, as in other vertebrates, thyroid hormones (THs) act on many biological processes including growth and reproduction. Primary THs such as thyroxine (T) and triiodothyronine (T) are known for their direct action on osmoregulatory organs regulating ion osmotic homeostasis in many teleost fishes. However, it is unclear how these hormones interact with estradiol-17β (E), an ovarian hormone that regulates the development of oocytes.