Colocalization of prolactin and growth hormone within specific adenohypophyseal cells in male, female, and lactating female rats.

The localization of PRL and GH within adenohypophysial cells has been investigated with immunocytochemical methodology using colloidal gold of different sizes. Classically, using morphological criteria at the light and electron microscope levels, two types of individual cells have been described which, it is believed, exclusively produce either PRL or GH, i.e.

Effect of hypothalamic deafferentation on hypophysial and other endocrine gland blood flows.

The effects of partial or complete deafferentation of the medical basal hypothalamus (MBH) on blood flow through endocrine glands was evaluated using a modification of Sapirstein's indicator fractionation method. Adult female Wistar rats were distributed into three groups consisting of normal control animals (n = 12), animals in which rostral deafferentation (Rd) of the MBH was performed (n = 11), and animals in which complete deafferentation (Cd) of the MBH was done (n = 6). Three weeks after the surgical procedures, Cd reduced adenohypophysial weight by 30% and raised its blood flow levels by 80% compared to those values in the Rd and control groups.

Use of immunoperoxidase and immunogold methods in studying prolactin secretion and application of immunogold labelling for pituitary hormones and neuropeptides.

Two immunocytochemical methods, immunoperoxidase and immunogold (IG), were used in an attempt to study the dynamic process of prolactin release from stimulated rat pituitary mammotrophs. The immunogold method was also used to localize other pituitary hormones including growth hormone, follicle-stimulating hormone, luteinizing hormone, and the neuropeptides substance P, neuropeptide tyrosine, leu-enkephalin, and atrial natriuretic factor in peripheral nerves. Light-microscopic immunoperoxidase staining of prolactin revealed a unique distribution of immunoreactive mammotrophs.

Complete suppression of plasma follicle-stimulating hormone in castrated male and female rats during continuous administration of porcine follicular fluid.

The effects of continuous i.v. infusion of porcine follicular fluid (PFF), presumed to contain inhibin activity, on plasma gonadotropin levels were investigated in adult male and female Sprague-Dawley rats.