A μSPEed microextraction combined with ultra-performance liquid chromatography (UHPLC) with UV detection was developed for analysing six veterinary antibiotics (tetracycline, chlortetracycline, oxytetracycline, doxycycline, sulfamethoxazole, and trimethoprim) in environmental samples. To optimise extraction, 12 sorbent cartridges, sample loading cycles, volumes, and pH were assayed. The PS/DVB-RP cartridge, three 250 μL sample loading cycles, and two 50-µL elutions with acidified methanol yielded maximum efficiency.
View Article and Find Full Text PDFBackground: Isometric and dynamic tasks of low-to-moderate intensities have been used to study sex differences in fatigability; however, maximal exertions with flywheel devices (FDs) have not been used. This study aimed to (1) detect sex differences in fatigue-related performance in a maximal intermittent fatiguing protocol on a FD, and (2) investigate the most sensitive dynamometric and mechanical variables for assessing fatigue in both sexes.
Hypothesis: No sex differences should exist when performing this protocol on a FD.
This work proposes a sustainable sequential extraction of bioactive terpenoids and phenolic compounds from grapefruit, lime, and lemon peels using supercritical CO extraction (SC-CO) and ultrasound-assisted extraction (UAE) with natural deep eutectic solvents (NaDES). NaDES screening demonstrated that Choline Chloride:Tartaric acid (1:2) for grapefruit and lemon peels, and Choline Chloride:Glycerol (1:2) for lime peels with 50% water yielded the highest phenolic contents. Cryogenic grinding (CR) and SC-CO pretreatments before UAE-NaDES did not improve phenolic recovery compared to direct UAE-NaDES.
View Article and Find Full Text PDF50% of pharmaceuticals and 25% of herbicides used worldwide are chiral. Each enantiomer has a unique toxicity and biodegradation profile, affecting differently to organisms. Chirality plays a key role in the behavior of these emerging contaminants (ECs) in terms of their pharmacological or herbicidal activity, but this peculiarity is often overlooked in environmental research.
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