Publications by authors named "M B Boniotti"
Porcine Reproductive and Respiratory Syndrome (PRRS) causes huge economic losses to pig farms worldwide. Currently available vaccines do not always offer complete protection, due to the extreme variability of the virus. Therefore, good farming practices must be improved to prevent the disease from spreading across the pig production system.
View Article and Find Full Text PDFLagoviruses are viruses of the Caliciviridae family affecting lagomorphs. Both pathogenic and non-pathogenic lagoviruses affect the European rabbit (Oryctolagus cuniculus), and they are phylogenetically distinguished. Rabbit Hemorrhagic Disease Virus (RHDV/GI.
View Article and Find Full Text PDFThe present study retrospectively analyzed the infectious agents associated with 829 abortion outbreaks occurring from 2011 to 2021 in northern Italy. Foetuses were subjected to necropsies, and organ samples were analyzed by direct PCR to screen for six swine pathogens. In 42.
View Article and Find Full Text PDFArticle Synopsis
- - Bovine tuberculosis (bTB) is a serious infectious disease affecting both livestock and wildlife, which can also be transmitted to humans, prompting a study in Italy to investigate its genetic makeup and transmission.
- - Whole-genome sequencing (WGS) of two common bTB strains in Italy revealed distinct genetic variations, with Italian isolates showing significant differences from other European strains, highlighting regional differences in infection.
- - The study found that while there’s limited genetic diversity within specific cattle herds, persistent strains are common in hotspots, underscoring the need for updated control strategies that leverage WGS and promote a One-Health approach to tackle this public health issue.
Bacteriological culture and direct PCR for detecting the Mycobacterium tuberculosis complex in the Italian eradication campaign: a decade of experience at the National Reference Laboratory.
J Appl Microbiol
March 2024
Aims: Our study evaluates the capacity of direct real-time PCR for detecting Mycobacterium tuberculosis complex (MTBC), with a focus on diagnostic performances and the feasibility of implementing this protocol in an eradication campaign. Specifically, we compare the effectiveness of the direct PCR method to various culture systems used by the Italian National Reference Laboratory over the last decade to detect MTBC.
Methods And Results: Bovine tissue samples were routinely tested and analyzed for bovine tuberculosis (bTB) confirmation using microbiological culture (solid and liquid media), histopathological analysis, and a direct PCR assay targeting IS6110, an insertion sequence specific to the MTBC that is widely used for tuberculosis diagnosis.