Glutathione's role in mitigating cadmium stress in Pogostemon cablin: Insights from combined transcriptomic and metabolomic approaches.

Cadmium (Cd) pollution poses a growing threat to plant growth. Although glutathione (GSH) is recognized for its potential to mitigate Cd-induced stress, its specific effects on alleviating such stress in Pogostemon cablin (patchouli) remain unclear. This study investigated physiological parameters and enzymatic activities across four treatment groups: control (CK), cadmium stress (Cd), glutathione (GSH), and glutathione with cadmium stress (GSH+Cd).

Effects of Polyphenolic Extracts from Sumac, Pomegranate Peel, Indian Almond Leaves, Falsa, and Banana Bract on Calcium Oxalate and Brushite Crystallization In Vitro.

Nephrolithiasis, or kidney stone formation, is a widespread global health concern. This study examines the effects of polyphenolic extracts, along with their anthocyanin and non-anthocyanin fractions, from sumac fruit, pomegranate peel, almond leaves, falsa fruit, and banana bract on the crystallization of calcium oxalate (CaC2O4.nH2O) and brushite (CaHPO4 2H2O) in vitro.

Antioxidants and Antidiabetic Potential of Polyphenolic Fractions and Crude Extracts of Rhus Coriaria L. Fruit, Punica Granatum L. Peel, and Terminalia Catappa L. Leaves: In Vitro And In Vivo Evaluation.

Rhus coriaria (sumac) fruit, Punica granatum (pomegranate) peel and Terminalia catappa (Indian almond) leaves' extracts and their anthocyanin and non-anthocyanin fractions were assessed in vitro for DPPH radical scavenging, FRAP, human salivary amylase and Dipeptidyl Peptidase IV (DPP-IV) inhibitory potentials, as well as for their in vivo antidiabetic effects on High-Sugar High-Fat Diet (HSHFD) + streptozotocin (STZ) induced diabetic rats (8-weeks study), by assessing fasting blood sugar, 1hr- and 2 hr-Oral Glucose Tolerance Tests, serum insulin, Homeostatic Model Assessment (HOMA) analyses, serum creatinine, urea and Blood Urea Nitrogen. All extracts and fractions showed antioxidant (DPPH and FRAP) and enzyme inhibition activities (HAS and DPP-IV) and also suppressed STZ-effects in diabetic mice by increasing Superoxide Dismutase, Glutathione S-Transferase and insulin and decreasing HOMA2-IR, urea and creatinine, with sumac extract showing benefits even when administered prior to STZ. These findings suggest that all tested extracts, particularly sumac, possess significant antioxidant and antidiabetic potential.

Molecular epidemiology and characterization of antibiotic resistance of isolated from livestock population of Punjab, Pakistan.

Haemorrhagic septicaemia (HS) is an acute and life-threatening infection of livestock population caused by (), responsible for huge mortality, morbidity and production losses. The increase in antibiotic resistance is a growing concern, posing a significant threat to animals and public health. There is limited data on disease burden, serotypes, antibiotic susceptibility, and resistance gene profiles in Pakistan.

"First-In-Human" Totally Robotic Orthotopic Heart Transplant.

Importance: Traditionally, heart transplantation surgery requires a median sternotomy due to the perceived complexity of a large organ replacement. Whether an alternative minimally invasive approach to perform a successful heart transplant can be accomplished remains uncertain.

Objective: To develop a safe and effective technique for totally robotic heart transplantation DESIGN: Using a multidisciplinary team, technical timed training using the robot-assisted approach was accomplished on multiple cadavers.